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Abstract Topic: Biology

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A Suspected New Species Closed to Dysmicoccus finitimus Williams (Hemiptera: Pseudococcidae) Lives on Oil Palm (Elaeis guineensis Jacq)
A Zarkani1*, MB Kaydan2 and D Apriyanto1

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Corresponding Author
Agustin Zarkani

Institutions
1) Department of Plant Protection, Faculty of Agriculture, University of Bengkulu, Bengkulu, Indonesia.
2) Biotechnology Research Centre, Çukurova University, Adana, Turkey.

*E-mail: agustinzarkani[at]unib.ac.id

Abstract
Eleven species of Pseudococcidae scales are known to live on oil palm (Elaeis guineensis Jacq). Here we report an outbreak of a suspected new species which is morphologically closed to Dysmicoccus finitimus Williams 1994 (Hemiptera: Pseudococcidae) on oil palm in Bengkulu Province, Indonesia. Some features are known different in the number of cerrarii, type of macro lobes, and longest anal-lobe seta. In addition, the natural enemies associated with the species were also listed.

Keywords
Biodiversity, oil palm, palmae, scale insect, taxonomy

Topic
Biology

Link: https://ifory.id/abstract/6G8nzM7YyrNU


ABILITY OF GARLIC EXTRACT AND KAEMPFERIA GALANGAL INHIBIT ASPERGILLUS sp. ISOLATED FROM SARDINE FISH PEDETAN
Ni Made Ayu Suardani Singapurwa, I Putu Candra, I Nyoman Rudianta

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Corresponding Author
Ni Made Ayu Suardani Singapurwa

Institutions
Department of Food Science and Technology, Warmadewa University

Abstract
‘Pedetan- is one of the food products of the traditional Balinese fish sardine which is processed by the community in the Jembrana Regency of Bali Province. This study aims to determine the antimicrobial activity of garlic and kaempferia galangal in inhibiting the growth of Aspergillus sp. which can contaminate the sardine fish Pedetan, The study was conducted by isolating Aspergillus sp. from Pedetan and testing the inhibitory power of garlic and Kaempferia galangal The results showed that the extract of garlic and Kaempferia galangal can inhibit the growth of Aspergillus sp. From the results of SEM observations it was found that garlic and kaemfiera were able to damage the Aspergillus sp. Mycelia. compared to control treatment. Garlic extract and kaempferia galangal can inhibit the growth of Aspergillus sp. because they contains bioactive compounds that are antimicrobial.

Keywords
Aspergillus sp., Garlic, Kaempferia galangal

Topic
Biology

Link: https://ifory.id/abstract/vVYnCDG9L8jW


Abundance of Green algae in The Intertidal Zone, Karang Papak Coastal, West Java, Indonesia
Tri Cahyanto (a), Epa Paujiah (b*), Ingga Rinasti (a)

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Corresponding Author
Epa Paujiah

Institutions
a) Department of Biology, Faculty of Sciences and Technology, UIN Sunan Gunung Djati Bandung
Jl. AH. Nasution, No. 105, Cibiru, Bandung
b) Department of Biology Education, Faculty of Tarbiyah and Teacher Training, UIN Sunan Gunung Djati Bandung
Jl. Soekarno Hatta, No. 750, Gede Bage, Bandung
*epapaujiah[at]uinsgd.ac.id

Abstract
Green alge (Chlorophyta) is one of macroalgae who have much function such as ecologically and economically living in the intertidal zone. Purpose of this research want to know about abundance of green algae group in the intertidal zone, Karang Papak Coastal, West Java, Indonesia. The research was carried out in November 2018 every week (following moon phase) with transect methods. The results showed that the Karang Papak Coastal have 15 Species with genus of Ulva who have highest abundance than other group. Its can be caused by several factor such micro and macro climate (Temperate). This data can be use for complete data about Indonesian macrolagae.

Keywords
Abundance, Indonesia, Intertidal Zone, Karang Papak Coastal, Macroalgae,

Topic
Biology

Link: https://ifory.id/abstract/FJT4vmjUxMhw


ACCURACY OF MYOGLOBIN mRNA IN THE BRAIN OF NORMAL RATS (Rattus novergicus) USING PCR REAL TIME TECHNIQUE
Rini Puspitaningrum1*, Apriliana L.F1, DiahAsriyanti1, Erfan Handoko2

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Corresponding Author
Rini Puspitaningrum

Institutions
1Dept. of Biology, Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia
2 Dept of Physics. Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia

Abstract
Myoglobin is a cytoplasmic hemoprotein with an important role of maintaining oxygen homeostasis of tissues. The brain needs quite a high amount of oxygen to carry out its metabolism. Therefore, this study was conducted to detect the expression level of myoglobin mRNA in the brain of normal rats (Rattus novergicus).The study was conducted at the Biochemistry and Molecular Biology Laboratory of the Biology Major of the Faculty of Mathematics and Natural Sciences, UNJ from December 2010 to February 2011. Through 1.5% agarose gel electrophoresis, results show that the myoglobin band of 114bp was detected in the brain of normal rats (Rattus novergicus). Due to the positive results, there is a huge possibility that the myoglobin expressed in the brain of rats (Rattus novergicus) would be altered into protein during environmental conditions of low oxygen concentration.

Keywords
myoglobin mRNA, normal, brain, Rattus novergicus.

Topic
Biology

Link: https://ifory.id/abstract/rNcgQJ3fxqPy


AN EXPLORATIVE STUDY ON THE USE OF CONDOM IN DIRECT COMMERCIAL SEX WORKERS IN SANUR
Luh Gede Pradnyawati

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Corresponding Author
Luh Gede Pradnyawati

Institutions
Faculty of Medicine & Health Sciences of Warmadewa University

Abstract
Previous sudies on commercial sex workers reported that the number of commercial sex workers spreading throughout Denpasar city and its surroundings is 250 persons and 14% of them had been infected with HIV/ AIDS (Human Immunodeficiency Virus/ Acquired Immune Deficiency Syndrome). However, referring to the data in the City Health Service of Denpasar, the number of direct commercial sex workers throughout Sanur area in 2017 is 450 persons. Such number confirms that the risk of sexually transmitted infection among the direct commercial sex workers in the area is intensely high. Condom use is seemingly ruled out. Thus, by the present study, an explorative investigation is carried out to explore more about the condom use among the direct commercial sex workers in Sanur. The study applies qualitative research design. A purposive sampling technique was implemented in selecting 15 informants. The 15 informants included the direct commercial sex workers, pimps, and customers. These informants were provided with in-depth interviews. The results of data analysis showed that during sexual intercourses not all the direct commercial sex workers applied the use of condoms. The leading reason was the male partners disliked the condoms in the sexual intercourse. However, it is also found that economic factor had given rise to avoiding the condom use. It should be noted well that using condoms during sexual intercourse decreases the growing number of the direct commercial sex workers.

Keywords
Condom, Sexually Transmitted Infection, Commercial Sex Workers, Sanur

Topic
Biology

Link: https://ifory.id/abstract/g4X7pjWRdx2L


Analysis of Factors That Affecting Student Learning Difficulties Towards Physiology Anatomy Lesson at SMK Negeri 3 Tangerang (Case Study Class X of Cosmetology at SMK 3 Tangerang).
ETI HERAWATI and DIAH AYU HAPSARI PURNOMO.

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Corresponding Author
Eti Herawati

Institutions
Universitas Negeri Jakarta

Abstract
This study aims to determine factors that cause learning difficulties to anatomy and physiology subjects lesson by class X student cosmetology Programs at SMK Negeri 3 Tangerang in Academic Year 2016 with a survey method. The populations were 64 people from class X students and Data collection tool used is questionnaires. The trial questionnaires carried out by 25 class XI cosmetology respondents. Instrument analysis carried out on validity and reliability, with 40 statement items and reliability result r11 = 0.954. The results of this study showed that the factors affecting students difficulties mainly on internal factors that are motivational factors indicators with sub student 88% learning persistence factor and independent learning factor equal to 94%. While external factors are indicators of teacher attention factor, with teachers sub-factor appreciation to students difficulties by 84%, and the factor of teachers involvement in students activities is equal to 91%, showing that any increase in the value of a teacher attention every 101,286 can cause an 879 increase with learning difficulties showed the 6.1% test effect, coefficient determination while the remaining 93.9% is influenced by other factors.

Keywords
Factors of Learning Difficulties, Physiology Anatomy

Topic
Biology

Link: https://ifory.id/abstract/EvPgM4NdKkr9


Analysis of Sound Frequency Exposure at Growing phase of Chrysanthemum Sp. (Case Study: Exposure by Quran Recitation)
Liberty Chaidir, Lia Kamelia, Aditya Rahman

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Corresponding Author
Lia Kamelia

Institutions
Faculty of Science and Technology
UIN Sunan Gunung Djati Bandung

Abstract
Sound wave technology or Sonic Bloom technology has long been applied to plants. Sound waves affected the plants at different frequencies, sound pressure levels, presentation periods, and distances from sound sources. The aim of this research is to determine the effect of sound technology exposure on certain frequencies on the beginning of shoot growth, plantlet height, leaf number, and stomata opening width . The experiment was conducted by comparing chrysanthemum plants exposed to quran recitation (Surah Al-Fatihah) at an average frequency of 1237.8 Hz for 2 hours for 8 Weeks After Culture (WAC). The results showed that murattal and media interactions occurred in planlet height after 4 WAC and leaf number after 8 WAC. The exposure of murattal had affected on plantlet height after 2 WAC. The treatment influenced the number of leaves after 4 WAC, and the number of roots after 6 WAC. In testing the opening of the stomata, the leaves that given murattal al-Fatihah treatment, having a stomata opening wider than the leaves that were not treated (control). This study is the beginning of research to find the right frequency to stimulate growth in plants.

Keywords
Chrysanthemum Sp.;Frequency;Growth;Quran recitation;

Topic
Biology

Link: https://ifory.id/abstract/AgweKQWMjEFy


Analysis Quality of Bioplastic From Kombucha Celullulose for Fruit Packaging Material as Biodegradable Plastic
Anggita Rahmi Hafsari, Nia Nuraeni, Ade Kartika Apriliani

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Corresponding Author
Anggita Hafsari

Institutions
Departement Biology, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung. Jl. AH. Nasution No 105 Bandung, 40614

Abstract
The purpose of this research was to obtain information about the best quality of bioplastic fruit packed using kombucha cellulose which grow in green tea media (Camellia sinensis L.). This research was carried out with a trial method of Completely Randomized Design (CRD) 5 arrangements with 4 replications and a total sample of 20. By administering 1%, 2%, 3%, 4% and 5% glycerol. The statistical analysis used is Analysis of One-way Variance (ANOVA). The characteristics of Bioplastic packaging from kombucha cellulose with the approval of glycerol concentrations of 1%-5% (v / v) produced a combined average mean value of 0.015 mm, 0.02 mm , 0.025 mm, 0.027 mm and 0.045 mm. The tensile strength values obtained were 7.22 N / mm2, 3.27 N / mm2, 2.17 N / mm2, 1.80 N / mm2 and 2.15 N / mm2. The highest percent value was 73%,. While the highest solubility values were 50%. Application of kombucha cellulose as a bioplastic packaging in tomato fruit is acceptable as an alternative packaging fruit biodegradable material for fruit.

Keywords
Bioplastic Packaging Fruit, Kombucha Celullose, Camellia sinensis L.Biodegradable

Topic
Biology

Link: https://ifory.id/abstract/E3zhRXuwUGV8


Antibacterial Activities Test of Cajuput Leaf Waste Extract (Melaleuca cajuputi Powell) on Pathogenic Bacteria
Ukit (a), Ana Widiana (b*), Evi Rahmawati (b), Rizal M Hasby (b)

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Corresponding Author
Ana Widiana

Institutions
(a) Faculty of Tarbiyah and Teaching Programme. UIN Sunan Gunung Djati Bandung.
Jl. AH. Nasution No. 105. Bandung. West Java. Indonesia
(b) Faculty of Science and Technology. UIN Sunan Gunung Djati Bandung.
Jl. AH. Nasution No. 105. Bandung. West Java. Indonesia
*anawidiana[at]uinsgd.ac.id

Abstract
Cajuput plants (Melaleuca cajuputi Powell) and its waste are one of the plants that can produce essential oils. Cajuput plant essential oil has the largest compound component, 1.8 cineol which has the ability as an antibacterial to kill pathogenic bacteria. The purpose of this study was to determine the antibacterial activity of cajuput leaf waste extract against pathogenic bacteria and find out the category of inhibition zones formed from extracts of cajuput leaf waste. This study used a completely randomized design method. Cajuput leaf waste used has a shelf life of 0 months, 2 months and 4 months old. The pathogenic bacteria used consisted of 17 isolates, each treatment was repeated twice. The results showed that cajuput leaf extract had antibacterial activity known by the formation of inhibitory zones. The inhibitory zone in the extract of the 0-month leaf waste was greater than in the leaves extract of leaves of 2 months and 4 months. The diameter of the inhibition zone is at most 13 mm and at least 1 mm. With these results, it can be concluded that the antibacterial activity of eucalyptuscajuput leaf waste belongs to the category of weak to strong.

Keywords
Cajuput leaf waste, antibacterial, pathogenic, inhibition zone

Topic
Biology

Link: https://ifory.id/abstract/Gkrg2MheVYN3


ANTIBACTERIAL ACTIVITY OF Lannea coromandelica EXTRACT
Hartati, Syamsuddin, Hilda Karim, Halifah Pagarra

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Corresponding Author
Hartati Hartati

Institutions
Biology of Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar

Abstract
This research that aims for antibacterial activity Lannea coromandelica extract. The klika was extracted by wet and dry method using ethyl acetate and ethanol 70% that acquired 4 types of extract they are ethanol extract 70% of wet klika Kayu Jawa (KJBE70%), extract ethanol 70% of dry klika Kayu Jawa (KJKE70%), ethyl acetate extract of dry klika Kayu Jawa (KJKEA), and extract of wet klika Kayu Jawa (KJBEA). Extracted by maceration, dan then antibacterial acivity. The results from this research shown that all extracts have antibacterial activity to inhibited bacteria test growth (Escherichia coli, Staphylococcus aureus, Pseudomonas sp., and Vibrio parahaemolyticus.

Keywords
Lannea coromandelica, antibacterial activity

Topic
Biology

Link: https://ifory.id/abstract/ApUNhD7gudqt


Antibiotic resistance pattern among clinically relevant bacterial isolates in the General Hospital Sanjiwani Gianyar Bali: A retrospective study
Anak Agung Gede Indraningrat (a*), Sri Masyeni (a), Made Sila Darmana (a,b)

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Corresponding Author
Anak Agung Gede Indraningrat

Institutions
a. Faculty of Medicine and Health Science, University of Warmadewa,Jln Terompong 24,Denpasar-Bali, Indonesia 80235.
* anak.indraningrat[at]gmail.com

b. General Hospital Sanjiwani, Gianyar-Bali, Indonesia

Abstract
Antibiotic resistance is currently an emerging global threat that is triggered by misused and overused of antibiotics, which led to higher mortality and morbidity rate. Information of antibiotic resistance iscrucial to determine the ideal treatment for patients. Since these data is rather scarce in Bali, we performed antibiotic susceptibility profile of different clinical bacterial isolates in the General Hospital Sanjiwani Gianyar Bali based on retrospective data in 2018. The data included type of tested antibiotics, name of clinical bacterial isolates, sex of patient and antibiotic resistance profile. Among 65 clinical bacterial isolates, high diversity of bacterial pathogen was found and grouped into 22 species, which Escherichia coli, Klebsiela pneumoniae and Staphylococcus aureus were the three predominant isolates identified. Exposure against 28 different antibiotics showed majority of bacterial isolates were resistant against baxtrim, aztreonam, ciprofloxacin, but susceptibility towards meropenem, amikacin and cefoperazone-sulbactam were observed. Routine surveillance to determine antibiotic resistance profile are of important and recommended to generate a local antibiogram as guideline to overcome pathogenic infections.

Keywords
Antibiotic resistance, susceptibility, clinical bacteria

Topic
Biology

Link: https://ifory.id/abstract/uRXfDJFP8KkH


Antimicrobial activity of Aspergillus sp fungi isolated from land waste disposal in Bogor, Indonesia on the growth of Staphylococcus aureus, Salmonella thyphosa and Candida albicans.
Dalia Sukmawati1,2* and Novitha Noorhadiatiningsih3

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Corresponding Author
Dalia Sukmawati

Institutions
1) Biology Department, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia.

2) Universitas Negeri Jakarta Culture Collection (UNJCC), 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia.

3) Universitas Muhammadiyah Prof. DR. HAMKA, Klender.

Abstract
Aspergillus sp. mold is found in various habitats, especially on the ground. Land waste disposal is a good medium for the growth of microorganisms due to the condition of the land that is rich in organic matter. This study is expected to provide information about the potential of Aspergillus sp. mold mainly from soil which can produce antimicrobial compounds. This study aims to determine whether the metabolic metabolites of Aspergillus sp. obtained have activities as antimicrobials against Staphylococcus aureus, Salmonella thyphosa and Candida albicans. The fermentation was carried out with a static fermentation method which was incubated for 5 days and the antimicrobial activity test was carried out by the diffusion method to use cylinders with 3 replications. The results of macroscopic and microscopic isolation and identification obtained five isolates from genera of Aspergillus sp. which were coded N1, N2, N3, N4 and N5. The metabolites were obtained from the fermentation of each fungus isolate Aspergillus sp. The test results of the metabolite activity of fungus Aspergillus sp. had antibacterial activity, against Staphylococcus aureus, Salmonella thyphosa and Candida albicans.

Keywords
Antimicrobial, Aspergillus sp., Static Fermentation.

Topic
Biology

Link: https://ifory.id/abstract/wHxyugzpNvEM


ANTIOXIDANT ACTIVITY FROM ETHANOL EXTRACT OF KUPA LEAVES (Syzygium polycephalum (Miq.) Merr & L. M. Perry) USING DPPH (2,2- diphenyl-1-picrylhydrazil) METHOD
Farid Perdana1*, Isye Martiani1, Dhanti M A1

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Corresponding Author
Farid Perdana

Institutions
1. Program Studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam Univesitas Garut Jl. Jati No. 42 B Tarogong Garut, Jawa Barat
*farid[at]uniga.ac.id

Abstract
Kupa plant is one of the endemic plants of Indonesia which is included in the tribe of guava (Myrtaceae). This study aims to determine the antioxidant activity of ethanol extract of kupa leaves (Syzygium polycephalum (Miq.) Merr & L. M. Perry), and to determine the class of metabolites available in simplicia and ethanol extract of kupa leaves. Extraction of kupa leaf simplicia was carried out by maceration method using ethanol 96%. The antioxidant activity test was carried out qualitatively and quantitatively. The qualitative antioxidant test was carried out by Thin Layer Chromatography using the developer chloroform: methanol: acidic format (8:2:1) and using a sitroborate and DPPH 0.2% spotting appearance. Quantitative testing of antioxidant ethanol extract of Kupa leaves was carried out using the DPPH method with vitamin C as a comparison. The TLC test results obtained a greenish color on the sitroborat and yellow on the DPPH with an rf value of 0.381. In the antioxidant test the kupa leaf ethanol extract obtained an IC50 value of 10,327 ppm which is a very strong antioxidant. And secondary metabolites found in simplicia and ethanol extract of kupa leaves are flavonoids, saponins, steroids / triterpenoids, quinones and phenols.

Keywords
Antioxidants, kupa leaves, ethanol extract, DPPH.

Topic
Biology

Link: https://ifory.id/abstract/BKJyqPnMC7DE


Antioxidant Activity of Ethanolic Extract, n-Hexane Fraction, Ethyl Acetate Fraction and Water Fraction of Garut Orange Leaves (Citrus reticulata Blanco)
Faizah Min Fadhlillah, Wiwi Oktaviani and Ria Mariani

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Corresponding Author
Faizah Min Fadhlillah

Institutions
Garut University

Abstract
Antioxidants are compounds that are able to eliminate, cleanse, and resist radical effects. Antioxidants can stabilize free radicals by complementing the lack of electrons possessed by free radicals. Several studies have shown that citrus leaves of other spesies have been shown to have antioxidant activity. This study aims to determine the antioxidant activity of ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction of garut orange leaves (Citrus reticulata Blanco) using DPPH (2,2-Diphenyl-1-Picrylhydrazyl) method. Garut orange leaves were extracted with ethanol 96% by macerator apparatus. Concentrated extracts were fractionated by liquid liquid extraction method with n-hexane, ethyl acetate and water solvent. Furthermore, the ethanolic extract, n-hexane fraction, ethyl acetate fraction and water fraction of garut orange leaves were tested for antioxidant activity using the DPPH (2,2-Diphenyl-1-Picrylhydrazyl) method. The antioxidant activity test results showed that ethanolic extract of garut orange leaves had better antioxidant activity, followed by n-hexane fraction, ethyl acetate fraction and water fraction.

Keywords
garut orange leaves, antioxidant activity, DPPH

Topic
Biology

Link: https://ifory.id/abstract/Qew3VhRzpE8d


Antioxidant Capacity of Dillenia sp. Leaf Extract Against DPPH (1,1-Diphenyl-2picryl Hidrazil) Radical
Sri Rahayu, Isfi Zahara, Afifah, Krisna Arya Pambudi, Supriyatin

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Corresponding Author
Isfi Zahara

Institutions
Department of Biology, Faculty of Mathematics and Natural Sciences
Universitas Negeri Jakarta, Indonesia

Abstract
Simpor (Dillenia sp.) is a plant of the Dilleniaceae family. Simpor (Dillenia sp.) has bioactive compound which is flavonoids that act as antioxidant. The purpose of this study was to measure the antioxidant capacity of the 96% etahnolic simpor leaf extract. CRD (Complete Randomized Design) was applied with 3x5 factors. Extraction of leaf samples done by maceration methode using ethanol 96% with variations in maceration time 24 and 48 hours and series consentration of (10%, 15%, and 20%). The antioxidant capacity assay of simpor leaf series concentration (10, 50, 100, 150, dan 200) ppm using the immersion method of the DPPH (1,1-Diphenyl-2picryl Hidrazil) measure absorption at a wavelength ot 517 nm and compared with the powder of ascorbic acid. The result showed that the ethanol extract has flavonoid qualitatively and quantitatively. Furthermore it also has radical scavenging ability against DPPH (1,1-Diphenyl-2picryl Hidrazil).

Keywords
antioxidant capacity, DPPH, leaves simpor, (Dillenia sp.)

Topic
Biology

Link: https://ifory.id/abstract/J6xKBPmrWg4w


Antiretroviral therapy substitution among HIV/AIDS patients visiting Sanjiwani Hospital, Bali
Sri Masyeni, IDG Wedha Asmara

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Corresponding Author
Dewa Ayu Putri Sri Masyeni

Institutions
Sanjiwani Hospital/Faculty of Medicine and Health Sciences, University of Warmadewa

Abstract
Abstract Background: Substitution of long-term ART exposed to HIV/AIDS patients are common due to the ART adverse event. The study aim is to describe the reason of ART substitution among HIV/AIDS patient at Sanjiwani Hospital Bali. Methods: A retrospective study has conducted to the medical records of HIV/AIDS patients at Sanjiwani hospital Bali, during 2006-1018. Clinical data retrieved from the medical records and presented as descriptive data. Results: Over a twelve-year period, 1112 HIV/AIDS patients evaluated in the study. The ART regimen were Zidovudine-based ART, Tenofovir-based ART and Stavudine-based, for 12.2%, 87.3%, 0.5%, respectively. There were 2.2 % switching of ART during the study periode. The most reason was anemia (48%), followed by reducing of kidney function (28%), allergic reaction (16%), and 4% of nausea and suspected clinically failure to ART. Conclusion: We highlight that anemia is the main reason of ART substitution among HIV/AIDS patients

Keywords
ART; substitution; HIV/AIDS

Topic
Biology

Link: https://ifory.id/abstract/qh27jCe3TwWU


Application of biochar and compost to changes in physical and chemical properties of soil and corn yield on dry land
I Gusti Lanang Agung Alit Adi Putra, Yohanes Parlindungan Situmeang *, Made Sri Yuliartini, and I Gusti Bagus Udayana

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Corresponding Author
Yohanes Parlindungan Situmeang

Institutions
Agriculture Faculty, Warmadewa University, Jl. Terompong 24 Tanjung Bungkak, Denpasar-Bali, 80235, Indonesia
* Email: ypsitumeang63[at]gmail.com

Abstract
The purpose of this study was to determine the effect of biochar and compost fertilizer and their interactions on changes in the physical and chemical properties of soil and corn yields on dry land. This experiment used a randomized block design with factorial patterns. The treatment consists of two factors, namely: the dose of biochar and compost. The first factor, the dose of biochar consists of 4 levels, namely: 0 tons ha-1, 5 tons ha-1, 10 tons ha-1, 15 tons ha-1. The second factor, the compost dosage consisted of 2 levels: 0 tons ha-1 and 20 tons ha-1. The highest dry shelled seed per ha was obtained from the treatment of 10 tons ha-1 biochar which was 9.21 tons which increased by 35.56% compared to the lowest dry shelled seed per ha obtained from the treatment without biochar which was 6.79 tons . The treatment of compost doses of 20 tons ha-1 gave the highest value of dry shelled beans per ha, which was 8.73 tons, increasing by 16.58% compared to the lowest yield of dry shelled seeds per ha, which was 7.49 tons in the treatment without compost.

Keywords
bamboo biochar, compost, soil physical and chemical properties, corn plants

Topic
Biology

Link: https://ifory.id/abstract/LPCMjcTunvkQ


Application of Killer Yeast Derived from Phylloplane Bintaro Leaf as a Biocontrol Agent for Destructive Molds in Postharvest Fruits on Malang Apple
Dalia Sukmawati1,2, Andisa Shabrina1, Iman Hidayat3, * Hesham El Enshasy4,5

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Corresponding Author
Dalia Sukmawati

Institutions
1) Biology Department, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia
2) Universitas Negeri Culture Collection, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia
3) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Indonesia.
4) Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), 81130 UTM, Skudai, Malaysia
5) Department of Bioprocess and Polymer Engineering, School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia

Abstract
Mold is one of causal agents of postharvest diseases in apples. These include Aspergillus sp., Penicilium expansum, Botrytis cinerea, and Venturia sp. Fungicide application in controlling postharvest diseases causing several problems such as fungal resistance, environmental pollution and health. Therefore, it is important to seek alternative solution which is safe to human and environment, such as biocontrol. The aims of this study were to examine biocontrol activity of yeast isolated from bintaro leaves in inhibiting the growth of fungal postharvest diseases in apples and to determine the identity of these yeasts by using molecular phylogenetic analysis of nucleotide sequencing from the ITS rDNA region. Samples of apples were collected from Perumnas Traditional Market, Klender, East Jakarta. Isolation was done by using direct isolation techniques. A total of 18 fungal isolates have been isolated from symptomatic apples. Pathogenicity assay showed that A. brasiliensis sensu lato strain A1 (KP value 100%; KeP 50%) and A. flavus sensu lato strain A17 (KP value 100%; KeP 31.25%) exhibit highest activity in deleterious apple fruits. Rhodotorula mucilaginosa strain T1 and two isolates of Aureobasidium c.f. pullulans (strain T3 and T4) showed highest antagonism activity against the fungal pathogens. Biocontrol assay showed that yeast isolates were capable to reduce the growth of A. brasiliensis and isolates T1 and T3 were capable to reduce the growth of A. flavus.

Keywords
Apple, Aureobasidium pullulans, biocontrol, fungi, Rhodotorula mucilaginosa

Topic
Biology

Link: https://ifory.id/abstract/D2H4TbuFxVcJ


Application of Lake Sludge and Natural Phosphate on Spinach Plant Cultivation (Amaranthus tricolor L.)
Ida Bagus Komang Mahardika (a*), I Gusti Made Arjana (a), Evaristus Odi Nango (a)

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Corresponding Author
Ida Bagus Komang Mahardika

Institutions
a Department of Agrotechnology, Faculty of Agriculture, Warmadewa University, Denpasar, Indonesia
* Email: gusmahardika62[at]gmail.com

Abstract
Lake sludge and natural phosphate can be applied to the cultivation of various plants, but have not been applied to spinach cultivation. The aim of the study was to determine the effect of Lake Sludge dosage and Natural Phosphate dosage on growth and yield of unripe spinach. The experimental method with factorial RCBD used on this research. The results showed that the interaction between treatment of Lake Sludge (D) and Natural Phosphate (P) dosages had no significant effect on all observed variables. The Lake sludge dose of 150 g per polybag gives total oven dry weight per plant of 8.07 g or 61.1% higher compared to a dose of 0 g per polybag (D0). Natural Phosphate treatment has a very significant effect on the total oven dry weight variables per plant. Natural Phosphate doses of 40 g per polybag provide total oven dry weight per plant of 7.31 g or 39.5% higher than a dose of without Natural Phosphate (P0). Both of the regression analysis between the dosages of Lake Sludge and Natural Phosphate with total oven dry weight per plant there is a linear relationship.

Keywords
Lake Sludge; Natural Phosphate; spinach

Topic
Biology

Link: https://ifory.id/abstract/7Qxwm9puDFeP


Association between physical fitness with dysmenorrhea among junior high school student
Ni Putu Ana Novita, I Made Sarmadi

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Corresponding Author
Dewa Ayu Putri Sri Masyeni

Institutions
Faculty of Medicine and Health Sciences, University of Warmadewa

Abstract
Dysmenorrhea or stomach pain every menstruation is common symptom of adult women. Primary dysmenorrhea usually occurs about 2 years after menarche and which age about ±20 years. There are many incidence of dysmenorrhea. Physical fitness is one of the factor that affect the occurrence of dysmenorrhea. This aims of the study to determine the percentage of dysmenorrhea and correllation to physical fitness. The method of this study was csross-sectional. The study was conducted 71 female people of SMP Negeri 3 Gianyar. The physical fitness was measured using Tes Kesegaran Jasmani Indonesia (TKJI) and dysmenorrhea data using questionnaire. The data was analysed using Somersd tes. The result was follow the physical fitness with very low category 6 (8,5%), low category 36 (50,7%), average category 25 (35,2%) and good category 4 (5,6%). The percentage of dysmenorrhea was 56 (78,9%) and not dysmenorrhea was 15 (21,1%). The result of this study indicated there was significan correllation between physical fitness with dysmenorrhea (p<0,005).

Keywords
Dysmenorrhea, Physical Fitness

Topic
Biology

Link: https://ifory.id/abstract/FAVTaBtPWbmx


Attack Mealy bug (Hemiptera: pseudococcidae) on onion wood plant (Protium javanicum burm.) In the university of Bengkulu
S Ginting1*, Nadrawati2, Agustin Zarkani2 and EK Depari3

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Corresponding Author
Sempurna Ginting

Institutions
1,2 Department of Plant Protection, Faculty of Agriculture, University of Bengkulu. Bengkulu 383711, Bengkulu, Indonesia.
3 Department of Forestry, Faculty of Agriculture, University of Bengkulu. Bengkulu 383711, Bengkulu, Indonesia.

W.R. Supratman road, Kandang Limun, 383711, Bengkulu, Indonesia
*sempurna[at]unib.ac.id

Abstract
Onion wood is one of the commodity timber which is widely used as carpentry wood and furniture by the people of Bengkulu. Mealy bug is a pest that attacks onion wood. The purpose of this study was to evaluate the attack of mealy bug pests on onion wood plants in the forest of the campus university of Bengkulu. The study was conducted with a purposive sampling survey, sampling plants, consisting of the nursery, sapling, pole and tree phases. The results showed that mealy bug can attack all phases of onion wood growth, namely from the seedling, sapling, pole and tree phases. The population of mealy bug is highest in the pole phase, compared to other phases and the dominant mealy bug are found in the leaves. Natural enemies was found predators of the Order Neuroptera: Chrysopidae, and mites. This information is expected to be a reference in developing strategies to control mealy bug pests on onion wood plants.

Keywords
Attack, mealy bug, natural enemies, Protium javanicum

Topic
Biology

Link: https://ifory.id/abstract/GDTzAHp7k3Bd


BAGGASSE CHARCOAL OPTIMATION BASED ON DIFFERENT CONCENTRATION AND IMMERSING TIME : IT USE IN STABILIZING COOKING OIL QUALITY
Sri Rahayu, Farid Pujiono, Maharani Dewi, Arief Prasetiyo

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Corresponding Author
Farid Pujiono

Institutions
Department of Biology, Faculty of Mathematics and Natural Sciences
Universitas Negeri Jakarta, Indonesia
Coresponding author: srirahayu[at]unj.ac.id

Abstract
Sugar cane is a raw material for making sugar which can only be grown in tropical regions. Sugarcane bagasse generally consist of cellulose sugar groups such as hemicellulose, lignocellulose, and lignin. other than that it contains hydroxyl groups to absorb colors and free fatty acids. The purpose of this study was to measure baggase charcoal optimation based on different concentration and immersing time to stabilize cooking oil quality. CRD (Complete Randomized Design) was applied with 3x3 factors. Baggase charcoal was activated using KMnO4 1N for 2 days. Variation of baggase charcoal concentration are (5%, 10%, 15%) and variation time to optimation for 4, 5, and 6 days. The result showed that baggase charcoal affected to decrease FFA (Free Fatty Acid). Furthermore it also affected with the turbidity of used cooking oil.

Keywords
sugar cane, cellulose, hydroxyl, free fatty acid

Topic
Biology

Link: https://ifory.id/abstract/j6uteYEfavyK


Balance of C (Carbon) and N (Nitrogen) of Bioslurry Fertilizer and Fertilization Dose on Leek Growth (Allium fistulosum)
Pri Riznaya, Ana Rochana, Mansyur

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Corresponding Author
Pri Riznaya

Institutions
Universitas Padjadjaran

Abstract
The purpose of the research was to know the effect of C / N balance of bioslurry fertilizer (biogas sludge) and fertilizer dose on the growth of leek (Allium fistulosum). The design used was a complete randomized design of factorial pattern. The first factor of the C and N balances are 18, 20, 30 and 40. The second factor is the composting dose of 10 tons / hectare, 20 tons / hectare and 30 tons / hectare. Each treatment was repeated three times. The observed variables were the increase in length, width and number of strands. The data obtained were analyzed variance (ANOVA). The result of ststistic analysis shows that there is C / N influence on the number of strands; there was a dose effect on the length and width of leek but no interaction between C / N and the dose of fertilization. The best onion growth occurs at a dose of 10 tons / hectare as it leads to an increase in length, the highest width which is an important indicator of onion measurements.

Keywords
C and N Balance, dose, leek, length increase, width and height.

Topic
Biology

Link: https://ifory.id/abstract/3x4L8udAYvz2


Benthos Population in Seagrass Ecosystem at Bira Island Kepulauan Seribu, Jakarta, Indonesia
Ratna Komala (a*), Mieke Miarsyah (a), Ratna Dewi Wulaningsih (a)

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Corresponding Author
Ratna Komala

Institutions
a) Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Indonesia

Abstract
One group of organisms that can be used as bioindicators of water quality is benthos, these organisms are spread over several habitats and also in coastal areas, one of which is in the seagrass ecosystem on Bira Island, located in the Kepulauan Seribu Jakarta. The aim of this study was to determine the dynamics of benthic population as an illustration of the environmental conditions on Bira Island for 6 months of observation. The method used in this study was descriptive with survey design. Data collecting by purposive sampling with the transect method at 3 observation stations. The data were analyzed descriptively through the calculation of abundance, diversity and dominance. The results of the study identified 23 species of benthos included in the 3 phylum with the largest to lowest composition represented by molluscs, arthropods and echinoderms phylum. The benthic population fluctuates and shows a pattern of decreasing trends every month during observation. The abundance of the highest species of each phylum is represented by Turicula javana (Mollusc), Portunus sp (Arthropods) and Holothuria atra (Echinoderms. The Species Abundance based on the observation station shows that the highest abundance is at station 1, followed by station 2 and the lowest is station 3. Benthos diversity at each station is in the moderate category, and there is no dominant species

Keywords
Benthos; Bira Island; Seagrass; Kepulauan Seribu

Topic
Biology

Link: https://ifory.id/abstract/gKb4dhqMWTzZ


Betung Bamboo Shoot (Dendrocalamus asper Backer ex Heyne) Extracts: Accelerating the Growth of Java Apple (Syzygium samarangense) Stem Cuttings
S Mulyaningsih, D Sumiati, H Hernawan, Robbiyani, Sriwahyuningsih

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Corresponding Author
Sri Mulyaningsih

Institutions
Institut Pendidikan Indonesia Garut

Abstract
ABSTRACT. The pruning waste of Java apple (Syzygium samarangense) tree branch can be used for its propagation by stem cuttings. The rooting process can be accelerated with the help of growth regulators, one of which is betung bamboo shoot extract. Using a randomized block design, this experiment aims at examining the effect of betung bamboo shoot extract with concentrations of 0%, 10%, 20%, 30%, 40% and 50% on the growth of Java apple stem cuttings. The samples were 48 stem cuttings with a diameter of 1 cm and a length of 15 cm. The results showed that betung bamboo extracts could accelerate the propagation process. The optimum concentration was 50%.

Keywords
Keyword: Java apple stem cutting, betung bamboo shoot extract, propagation acceleration.

Topic
Biology

Link: https://ifory.id/abstract/YtwukxJK3BbF


Biological studies, growth patterns and maturity levels of gonad fish cork (channa striata) living on river and irrigation channel
I Putu Suardika, Dewa Gede Semara Edi, I Wayan Arya, Ni Made Darmadi *

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Corresponding Author
Ni Made Darmadi

Institutions
Water Resources Management Study Program, Faculty of Agriculture, Warmadewa University, Denpasar, Bali
*Email: nimadedarmadi[at]gmail.com

Abstract
This study aims to determine the growth pattern and maturity level of gonad cork fish living in Tukad Yeh Sungi and Subak Baru irrigation channel. This research uses observation method, descriptive and analytic method. Sampling method directly catch fish in the cork by using fishing gear then analytic and described. The samples counted 101 fish samples. Using analytical and descriptive data analysis techniques. The results of the study found the growth pattern of cork fish in negative alometric river while in the irrigation channel for the positive allometric female and the negative alometric male.The maturity level of gonad of cork fish seen directly from the surgical result is influenced by the weight and weight of the fish weight.

Keywords
Biological Study, Growth Pattern, Gonad Maturity Level

Topic
Biology

Link: https://ifory.id/abstract/UAzHGJMaFrgn


Biomonitoring of Cilaja River based on benthos community, in Ujung Berung Bandung within 2013-2017
Hertien Koosbandiah Surtikanti

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Corresponding Author
Hertien Koosbandiah Surtikanti

Institutions
Departemen Pendidikan Biologi
Program Studi Biologi, FPMIPA UPI

Abstract
Water catchment area of Cilaja is located in along Giri Mekar and Sindanglaya village, Ujung Berung, Bandung. This river has high values for the people living surrounding that area. It supports clean water supply, coffee plantation, agriculture area and fish farming area. Field study is done to monitor water quality due to high variation of human activities. Three location sites (coffee/pinus plantation, agriculture and residential area) were selected. Bioindicator benthos were collected from these site locations. Chemical-physical water were measured and analysed. This biomonitoring study is done between year 2013-2017. The results show that based on the distribution and composition of bioindicator benthos, the three differrent land uses has good water quality within 5 years. People attitudes in environmental aware play an important role in conserving water quality for long period of time.

Keywords
water quality, bioindicator benthos, human activities, chemical water

Topic
Biology

Link: https://ifory.id/abstract/uwevGEF3tUkh


Biostratigraphic Analysis Based on Planktonic Foraminifera from Well X in North Sumatra Basin
Dewi Syavitri, Romy Setiawan, M. Ali Jambak

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Corresponding Author
Dewi Syavitri Husein

Institutions
Geology Department
Faculty of Earth and Technology
Trisakti University

Abstract
The research area is in the X Well which located in the North Sumatra Basin. There have been several studies conducted in the area, but there is still very little information related to the age of the Formations in the basin. A total of 128 sample are used for lithostratigraphy analysis, in which 33 samples were analyzed further for the Foraminifera biostratigraphy study from the X Well at a depth of 6090 – 9919 feet. From the result of the analysis, 38 species from 16 genera successfully identified, as well as 5 datum species, namely Globigerinoides sicanus, Orbulina suturalis, Globorotalia menardii, Globorotalia plesiotumida dan Globorotalia tumida tumida. From the lithology data, three (3) lithostratigraphic unit successfully identified, namely the calcareous claystone, very fine calcareous sandstone and fine calcareous sandstone unit which is estimated to be comparable to the Baong, Keutapang and Seurula Formation. From the result of the study it-s concluded that the lithostratigraphic units found in the study area have the age range of N7-N18 or late part of the Early Miocene to the late part of the Late Miocene.

Keywords
Biostratigraphy, planktonic foraminifera, North Sumatra Basin.

Topic
Biology

Link: https://ifory.id/abstract/RMtXTuLfxkZA


BIOSURFACTANT- PRODUCING AND ANTHRACENE-DEGRADING BACTERIA FROM OIL CONTAMINATED SOIL
Tri Handayani Kurniati (a*), Iman Rusmana (b), Ani Suryani (c), Nisa Rachmania Mubarik (d)

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Corresponding Author
Tri Handayani Kurniati

Institutions
a) Biology Study Program, Faculty of Mathematics and Natural Sciences, Jakarta State University.
Jl, Rawamangun Muka, Rawamangun,. East Jakarta 13220. Indonesia.
b) Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University.
Jl. Raya Darmaga, Bogor16680, West Java. Indonesia.
c) Department of Industrial Technology, Faculty of Agriculture Technology, Bogor Agricultural University
Jl. Raya Darmaga, Bogor 16680, West Java. Indonesia.
d) Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University.
Jl. Raya Darmaga, Bogor 16680, West Java. Indonesia.

Abstract
Anthracene, together with other polycyclic aromatic hydrocarbons (PAHs), is a persistent and toxic soil contaminant because its low water solubility and high hydrophobicity. The effectiveness of PAH degradation could be enhanced by using biosurfactant. In this study, we isolated bacteria that were able to degrade anthracene and also produce biosurfactant. Samples were taken from oil contaminated soil at Jakarta Bay. Isolation was done in a Minimal Salt Medium (MSM) supplemented with 1% crude oil. Screening methods for biosurfactant production include Blood Hemolysis, Drop Collapse Assay and Oil Displacement Assay. Three isolates which have biosurfactant production and anthracene degradation capability was identified based on 16SrRNA gene. These isolates were identified as Ochrobactrum intermedium AMA9, Ochrobactrum tritici CHA60, and Pseudomonas stutzeri CRA7. Based on GC-MS analysis, Ochrobactrum intermedium AMA9 showed high ability to degrade 84,79% anthracene in liquid MSM medium supplemented by 100 ppm anthracene after seven days incubation.

Keywords
anthracene; bacteria; biosurfactant; degradation

Topic
Biology

Link: https://ifory.id/abstract/nZCGH3FtjKkh


Blood gas Analysis of Bajau Sea Nomads Tribe Divers Indonesia
Rini Puspitaningrum1, Azzahra Humairrah2, Yanti Susianti3, Erfan Handoko4, Arina Khairunnisa3, and Azzahra Mutmainah5

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Corresponding Author
Rini Puspitaningrum

Institutions
1. Department of Biology, FMIPA Universitas Negeri Jakarta
2. Human Medicine, JLU Giessen Germany
3. Medical Doctor, UIN Syarif Hidayatullah Jakarta
4. Department of Physics, Universitas Negeri Jakarta
5. Department of Biological Educational, FMIPA Universitas Negeri Jakarta

Abstract
The Bajau sea nomads as a famous tribe Indonesia. Bajau can breathe in sea water more than 3 minutes. There have been studies conducted on the Bajau tribe before, but this research will discuss the analysis of the Bajau tribes blood gas. The 20 people from the Bajau Tribe agreed, after the interview was taken, blood would be collected to find out how much PaO2 was. It was found that all the Bajau tribes had a PaO2 yield of more than 95%, which indicated that the oxygen in their blood was very high. Average depth above 3 meters.

Keywords
Bajau sea nomads tribe, diving, breathing adaptation

Topic
Biology

Link: https://ifory.id/abstract/jWQCKrVevDuZ


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