Indonesia Conference Directory


<< Back

Abstract Topic: Microbiology

Page 1 (data 1 to 10 of 10) | Displayed ini 30 data/page

A TEST OF THE POTENTIAL OF AMYLOLYTIC AND CELLULOLYTIC BACTERIA FROM SOME LAKES IN EAST JAWA TO ACCELERATE THE GROWTH OF THE LIPID PRODUCING MICROALGAE
Sitoresmi Prabaningtyas1, a)Yanis Kurnia Basithoh2), Rika Ardila2), Endang Suarsini1)

Show More

Corresponding Author
Sitoresmi Prabanigtyas Prabanigtyas

Institutions
1) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jalan Semarang No. 5, 65145, Malang, East Java, Indonesia
2) Students of the Biology Undergraduate Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jalan Semarang No. 5, 65145, Malang, East Java, Indonesia

Abstract
Abstract. Bacteria and microalgae in the aquatic environment interact in various ways. Some bacteria have the potential to accelerate microalgae growth. Amylolytic and cellulolytic bacteria which living in freshwater environments (lakes) can decompose complex compounds into simpler ones that needed by microalgae. However, there is limited information about amylolytic and cellulolytic bacteria in Ranu Pani, Ranu Regulo, Ranu Grati and Lake Ngebel. This study aims to examine the potential of amylolytic and cellulolytic bacteria isolated from Ranu Pani, Ranu Regulo, Ranu Grati and Lake Ngebel. The amylolytic test was performed through planted the bacterial isolates from each lake on Amilum agar. Meanwhile, the cellulolytic test was performed using CMC (Carboxymethyl cellulose) media. Our recent finding from 4 lakes showed that 53 isolates had the potential to hydrolyze starch, and 47 isolates had the potential to hydrolyze cellulose. The ANOVA results showed there were differences significantly (p<0.050) in the hydrolysis ability of amylolytic and cellulolytic bacteria isolates. The bacteria with the highest amylum hydrolysis ability were PAS isolates with hydrolysis index 5.89. The bacteria with the highest cellulose hydrolysis ability were PSS isolates with hydrolysis index 6.912. Based on the analysis of 16S rRNA gene sequences, PSS isolates were Bacillus paramycoides and PAS isolates were Bacillus subtillis and the two bacterial were isolates from Ranu Pani.

Keywords
amilolytic, cellulolytic, bacteria, lake

Topic
Microbiology

Link: https://ifory.id/abstract/W4cqbQEZg8XG


Amylolytic Ability of Bacteria Isolated from The Sago Pulp of The Traditional Sago Industry in Palopo, South Sulawesi
Uswatun Hasanah (a*) , Tri Ardyati (a) , and Prilya Dewi Fitriasari (b)

Show More

Corresponding Author
Uswatun Hasanah

Institutions
a) Department of Biology, Universitas Brawijaya, Jl. Veteran 6, Malang 65144, East Java, Indonesia
*annahasanah2103[at]gmail.com
b) Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Maulana Malik Ibrahim Malang, Jl Gajayana 50, Malang, Indonesia

Abstract
Sago is a plant that is widely cultivated in Indonesia with a planting area of 1.128 million ha. Sago waste, especially sago pulp, has quite a lot of starch so that it can be used as an amylolytic bacteria substrate. Amylase enzyme that produced by bacteria have the potential to be applied in industrial sector, food, medicine, and also be used as an effort to utilize waste. This study aims to obtain amylolytic bacteria isolated from the sago pulp industry in Palopo, South Sulawesi and the ability to produce amylase enzyme. Samples were taken from sago pulp of traditional sago industry in Palopo, South Sulawesi and isolated by using selective media. Pure isolate then screened and observed the ability to produce amylase enzyme. The results were obtained 9 potential isolates with qualitative assay obtained the highest amylolytic index shown by isolates 2B (9.004mm), 2D (7.28mm), and 4A (4.67mm). Quantitative assay obtained the highest amylase enzyme activity test shown by isolate 2B (3.62 U/mL) indicated that isolates from sago pulp had good potential to produce amylase activity.

Keywords
Amylase activity, amylolytic bacteria, sago pulp

Topic
Microbiology

Link: https://ifory.id/abstract/rQJKqTgAZ7et


Antibacterial Activities of Curcuma mangga Val. Extract in some Solvents to Staphylococcus aureus and Escherichia coli
Bayyinatul Muchtaromah1, a), Evika Sandi Safitri1, b), Prilya Dewi Fitriasari1, c), Jujuk Istiwandhani1, d)

Show More

Corresponding Author
Bayyinatul Muchtaromah

Institutions
1 Department of Biology, Faculty of Science and Technology, The State Islamic University of Maulana Malik Ibrahim Malang, Jl Gajayana 50, Malang, Indonesia

Abstract
Curcuma mangga is one of the traditional medicine that has the effect of dealing with infertility problems. Staphylococcus aureus and Escherichia coli are bacteria that is often found as the cause of the female reproductive tract infection. The purpose of this study was to determine the antibacterial activity of C. mangga extract against S.aureus and E. coli in several solvents. C. mangga rhizome was extracted by a maceration method using ethanol (polar), chloroform (semi-polar), and n-hexane (non-polar) solvents. The antibacterial activity test against S.aureus and E. coli using Kirby Bauer method with 100% concentration and microdilution method with concentration (%) 50, 25,12.5, 6.25, 3.13, 1.56, 0.78, 0.39. The highest inhibition zone diameter for S.aureus was obtained by ethanol (10.11 m), chloroform (9.21 mm), n-hexane (6.05 mm) extract while for E. coli respectively achieved by ethanol extract (8.06 mm), n-hexane (5.88 mm) and, chloroform (4.19 mm). The MIC value of ethanol extract to S.aureus was found at concentrations of 3.13% and MBC at 6.25%. The MIC value of ethanol extract on E. coli was obtained at concentrations of 6.25% and MBC at 12.50%. Ethanol extract of C. mangga rhizome produced the most antibacterial activity than chloroform and n-hexane.

Keywords
antibacterial, chloroform, Curcuma mangga, Escherichia coli, ethanol, n-hexane, Staphylococcus aureus

Topic
Microbiology

Link: https://ifory.id/abstract/4j6XNFQctxeH


Effect of Differences in the Form of Photobioreactor Prototypes and Aeration Period on Chlorella sp Cell Growth in Co-Culture with Bacteria
Lirofiatillah (1), Sitoresmi Prabaningtyas (1.a), Murni Saptasari (1), Eko Agus Suyono (2)

Show More

Corresponding Author
Lirofiatillah Lirofiatillah

Institutions
(1) Department of Biology, Faculty of Mathematics and Natural Sciences,
State University of Malang, Jl. Semarang 5 Malang 65145, Indonesia
(2) Faculty of Biology, Gadjah Mada University, Bulaksumur, Caturtunggal, Kec. Depok, Kabupaten Sleman, Daerah Istimewa Yogyakarta 55281, Indonesia

Abstract
Microalgae are photosynthetic microorganisms that produce oil which have the potential for fuel production. Increasing the growth of microalgae can be done by co-culture. The purpose of this study was to determine the effect of photobioreactor prototype shape, aeration period and interaction between the aeration period and the photobioreactor prototype form on the growth of co-culture Chlorella sp cells with bacteria. This study uses a Completely Randomized Design (CRD). The form of the prototype used is the cube and horizontal plate. The treatment of different aeration periods consists of 6A: 18TA; 12A: 12TA; 18A: 6 TA and 24 A: 0 TA (A = Aeration, TA = No Aeration). The study was carried out by observing Chlorella sp cell density in co-culture with bacteria every 7 days. The results showed that the optimal prototype shape was a horizontal plate with an average cell density of 11.48x10 4 cells/ml. Horizontal plates are also often referred to as flat panels. This flat panel photobioreactor has a length and height and a narrow width to allow for greater penetration of light. Aeration serves to prevent the deposition of microalgae and the homogenization of the environment, so that all cells can obtain light and food, facilitate gas exchange between the cultivation and air environments. The optimal aeration period was 12 hours aeration: 12 hours not aerated with a mean cell density of 38.8 x10 4 cells/ml. The results showed that there was a significant difference in the treatment of prototype forms and aeration periods towards the growth of Chlorella sp cells co-culture with bacteria. The results also showed that there was no interaction between the different aeration periods and the photobioreactor prototype on the growth of co-culture Chlorella sp cells with bacteria.

Keywords
Prototype, photobioreactor, aeration, co-culture

Topic
Microbiology

Link: https://ifory.id/abstract/zNZYgRw9BA76


Endophytic culturable bacterial obtained from Cinchona ledgeriana Moens. in West Java and its antagonistic effects against Phytophthora infestans
F A Anugrah (1), R Masita (1), H O Rusdi (1)

Show More

Corresponding Author
Fauzi Akhbar Anugrah

Institutions
(1) Universitas Negeri Malang
Jl Semarang 5 Malang, Inonesia (65145)
E-mail: fauzi.akhbar.fmipa[at]um.ac.id

Abstract
Medicinal plants Cinchona (Cinchona Ledgeriana Moens.) was once an Indonesian export commodity many years ago. Unfortunately, the decline of Cinchona production is currently also affected by plant-damaging fungal (Phytophthora infestans). This study aims to obtain endophytic bacteria in healthy Cinchona plants. A number of 64 endophytic bacterial isolates were evaluated for their ability to inhibit Phytophthora mycelium growth by dual culture technique. Nine isolates had inhibitory activity against fungal mycelium growth above 50% compared to controls. The highest percentage of inhibition is shown by isolate B13 which is 84.31%. Many bacteria are motile and make calculations biased. Furthermore, an antagonistic confirmation test was carried out using a glass ring. Six of them still proved to be consistent in having the ability to inhibit mycelium growth respectively for isolates B13 was 68.32% ± 3.34, isolates D5 67.67% ± 0.92, isolates D13 were 65.43% ± 1.49, isolates B15 were 61.98% ± 1.18, isolates D11 were 58.91% ± 2.06, and B14 isolates at 57.76% ± 2.84. This result is a new literature in the study of the potential of Cinchona plant endophytic bacteria that can be developed for bio-control applications that are safe for plants and the environment.

Keywords
Endophytic bacteria, Cinchona, Dual culture, Antagonistic effect

Topic
Microbiology

Link: https://ifory.id/abstract/Rfe6DbHrX7TK


Molecular Characterization and Secondary Metabolite Profiling of Endophyte Bacteria from Purwoceng (Pimpinella pruatjan Molk.) Roots as Antibacterial Against Pseudomonas aeruginosa
Nur Aini Kartikasari 1,a), Yekti Asih Purwestri 2,b), Langkah Sembiring 2,c)

Show More

Corresponding Author
Nur-aini Kartikasari

Institutions
1 Department of Biology, Faculty Mathematics and Natural Sciences, Universitas Negeri Malang, Jalan Semarang No.5, Malang, 65145, Indonesia.
2 Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia.
a)Corresponding author: nuraini.kartikasari.fmipa[at]um.ac.id
b) yekti[at]ugm.ac.id
c) lsembiring[at]ugm.ac.id

Abstract
Study of endophyte bacteria from Purwoceng roots (Pimpinella pruatjan Molk.) lead to characterized endophyte bacteria that can produce the same secondary metabolite compound as its host. Secondary metabolite from this bacteria can be used as antibacterial against Pseudomonas aeruginosa. This research aims to determine antibacterial activity of secondary metabolites purwoceng root endophyte bacteria against P. aeruginosa growth, characterize the group of secondary metabolites produce by endophyte bacteria and identify potential endophyte bacteria using molecular character 16S rRNA gene sequence. Method used in this research such as antibacterial activity assay to determine potency of antibacterial compound produce by endophyte bacteria against P. aeruginosa growth, thin layer chromatography (TLC) assay to characterize the group of antibacterial compound and sequencing of 16S rRNA gene to determine the strain of potential endophyte bacteria. The result of bacterial activity showed GP2, GP14 and DG1 isolate produce antibacterial compounds by forming inhibitory zone in antibacterial activity assay. Antibacterial compounds produce by GP2, GP14 and DG1 isolate belong to alkaloid family which has the ability as antibacterial. Based on molecular identification GP14 and DG1 isolate has the similarity with Bacillus subtilis by 99%, while GP2 isolate has the similarity with Enterococcus faecalis by 99%.

Keywords
endophyte bacteria, purwoceng, antibacterial, 16S rRNA, P. aeruginosa

Topic
Microbiology

Link: https://ifory.id/abstract/uRMpvzPLK76G


The capacity of phosphate absorption and gibberellin level of bacterium Bacillus strain from peppers- rhizosphere
Masniawaty1, Kahar Mustari2 , Astuti3, Gusmiaty3, Siti Halimah Larekeng3, Iradhatullah Rahim4a)

Show More

Corresponding Author
Iradhatullah Rahim

Institutions
1 Department of Biology, Universitas Hasanuddin, Makassar
2 Department of Agrotehnology, Faculty of Agriculture, Universitas Hasanuddin, Makassar
3 Faculty of Forestry, Universitas Hasanuddin, Makassar
4 Agrotechnology, Faculty of Forestry, Animal Science, and Fishery, Muhammadiyah University, Parepare

Abstract
Microbes are capable to dissolve phosphate. Phosphate is the second essential component after N, which has a crucial role in photosynthesis and root development. A microbe that found around root area is the Bacterium Bacillus strain. This bacterium was isolated from peppers- roots. The purified isolates were quantitatively evaluated for their capacity to dissolve phosphate using Pikovskaya liquid medium with Ca3(PO4)2 as phosphate source. Meanwhile, production of gibberellin hormone was measured using Borrow et al (1995) method. Here, we reported levels of phosphate and gibberellin produced by the bacteria ranged from 19.0 up to 58.8 µg/L and 5.81 up to 12.79 µg/L, respectively. This results indicate that the Bacillus strain isolated from peppers- roots and root area can be utilized as plant growth-promoting bacterium.

Keywords
Bacillus strain, Gibberellin, Phosphate, Root area

Topic
Microbiology

Link: https://ifory.id/abstract/nYhtkHA8RaLE


The Diversity of Arbuscular Mycorrhizal Fungi Associated With Rambutan Tangkue Variety in Lebak Banten Province
Rida Oktorida Khastini, Indah Juwita Sari, Asep Sumantri

Show More

Corresponding Author
Asep Sumantri

Institutions
Universitas Sultan Ageng Tirtayasa

Abstract
Tangkue, one of rambutan (Nephelium lappaceum L) variety originated form Banten Province found to be mycotrophic. The aim of this study was to investigate the association of Arbuscular Mycorrhizal Fungi (AMF) and diversity in Rambutan Tangkue (Nephelium lappaceum L.) in Lebak Cilayang, Banten. This research was conducted from July 2018 to January 2019. The rambutan soil rhizosphere and roots were sampled from two stations and the AMF spores were trapping and extracted by Wet sieves and decantation techniques The results showed that the spore density Spore density varies between 2012-372/100g of soil. About four genera has been successfully isolated: Glomus, Acaulospora, Gigaspora and Scutellospora. Glomus is the most dominant genus in the Rambutan Tangkue rhizosphere.

Keywords
Rambutan tangkue, Arbuscular Mycorrhizal Fungi, diversity

Topic
Microbiology

Link: https://ifory.id/abstract/cjruBKxk3qFA


The number of Escherichia coli on several flies from settlement and garbage dump: Drosophila is the least!
Ainur Rofieq, Atok Miftachul Hudha, Elke Ayu Augista, Ahmad Fauzi*

Show More

Corresponding Author
Elke Ayu Augista

Institutions
Universitas Muhammadiyah Malang

Abstract
Flies are vectors of disease because flies can transfer bacteria from dirty places to food or the human body. The purpose of this study was to determine the differences in the number of Escherichia coli carried by three types of flies in different location (settlement and garbage) dump. This factorial study involved three types of flies, i.e. the house fly (Musca domestica), green fly (Chrysomya megacephala), and fruit flies (Drosophila sp.). Catching flies is carried out at 02.00 to 05.00 pm for four times a week. Calculation of the number of E. coli using the colony counter in CFU / ml units and two-way ANOVA was chosen to analyze the data that has been obtained. The result, differences in location did not give a significant effect (p = 0.533), while the type of fly had a significant effect on the number of E. coli (p < 0.001). The location and the type of fly also did not show a significant interaction (p = 0.816). Interestingly, Drosophila is the type of fly that carries the least amount of E. coli when compared to the other two types of flies.

Keywords
Chrysomya megacephala, Drosophila sp., Escherichia coli, Musca domestica

Topic
Microbiology

Link: https://ifory.id/abstract/3jmz6NwM7Rx4


Xylanase Enzyme Activity Produced from Aspergillus niger and Aspergillus oryzae Using Sugarcane Baggase and Wheat Bran Substrates
Uun Rohmawati, Nugrahaningsih, Agung Witjoro

Show More

Corresponding Author
UUN ROHMAWATI

Institutions
State University of Malang

Abstract
Xylanase enzyme is extracellular enzyme that plays a role in hidrolyzing xylan into short chains of xylose and xylooligosaccharides. The xylanase enzyme is one of the most important enzymes in various industrial sectors including the paper, textile and xylitol industries. Xylanase enzyme are produced by microorganisms such as bacteria, yeast and fungi with the help of xylan as an xylanase enzyme induction medium. The use of commercial xylan in industrial scale xylanase production is not economical because the price is too expensive $ 180-200/kg, so that an alternative is needed to use xylan from agricultural waste such as bagasse and wheat bran. Bagasse and wheat bran contain higher xylan compared to other agricultural wastes are 29.2% and 43.2%. This study aims to determine the optimal concentration in mixing bagasse and wheat bran substrates to the xylanase enzyme activity produced by Aspergillus niger and Aspergillus oryzae through the 2,4 dinitrosalicylic acid method. The test results of the xylanase enzyme activity of Aspergillus niger and Aspergillus oryzae showed that xylanase production increased with increasing concentration of wheat bran and decreased by adding concentration of bagasse. Based on the results of the enzyme activity test it can be concluded that the substrate which produces high xylanase enzyme at 100% wheat bran is 6.6 U / mL, while mixing the wheat bran and bagasse substrate which produces high xylanase activity at a concentration ratio of 75% wheat bran added 25% bagasse that is equal to 5.4 U / mL. The xylanase enzyme activity produced by Aspergillus oryzae was higher than Aspergillus niger which was 4.49 U / mL.

Keywords
Xylanase, Aspergillus niger, Aspergillus oryzae, sugarcane bagasse, wheat bran

Topic
Microbiology

Link: https://ifory.id/abstract/23HhTNeubdZw


Page 1 (data 1 to 10 of 10) | Displayed ini 30 data/page

Featured Events

<< Swipe >>
<< Swipe >>

Embed Logo

If your conference is listed in our system, please put our logo somewhere in your website. Simply copy-paste the HTML code below to your website (ask your web admin):

<a target="_blank" href="https://ifory.id"><img src="https://ifory.id/ifory.png" title="Ifory - Indonesia Conference Directory" width="150" height="" border="0"></a>

Site Stats