Analysis of Operating Variables For Microwave-Assisted Extraction of Pereskia Bleo Kunt DC Leaves Using Different Solvent
1 Wong, I.N.B.M.K.,1, 2,* Abd Gani, S.S., 3Halmi, M.I.E and 1,4Zaidan, U.H.

1. Halal Product Research Institute, Universiti Putra Malaysia, Putra Infoport, 43400 UPM Serdang, Selangor, Malaysia

2. Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
*Correspondence: ssalwaag[at]upm.edu.my

3. Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

4. Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

Abstract

Pereskia bleo (Kunt) DC is a leafy cactus species traditionally used by locals in Malaysia for treatments of various ailments known to be due to its high total phenolic content (TPC). There are a number of procedures in extracting TPC from plant materials. This paper reports a study conducted on maximizing microwave-assisted extraction of TPC, expressed as gallic acid equivalent (GAE), from leaves of the species using one variable at a time method (OVAT). The study establishes that the best solvent for extraction of TPC from leaves was ethanol with concentrations ranging from 20-40%. Solvent to solid ratio was set at 1:30. The best extraction time and microwave power to obtain high yield of TPC ranged from 30 to 60 seconds and from 40W to 50W, respectively. The study presents a simple and efficient non-conventional method of extraction of TPC from Pereskia bleo simplifying extraction procedure in an attempt at exploiting the potentials of the species as a source novel therapeutic agent. In the future, anti-inflammatory activities of Pereskia bleo will be investigated and the preliminary study on the extracts is for future halal and organic formulation.

Keywords: Pereskia bleo, one variable at a time, microwave-assisted extraction, total phenolic compound.

Topic: Halal Science and Technology

Link: https://ifory.id/abstract-plain/2VHeNhdWbkAq

Web Format | Corresponding Author (Intan Nur Balqis Mohd Khalid Wong)