Antibacterial Activity of Pisonia grandis Extract against Escherichia coli and Enterotoxigenic Escherichia coli (ETEC)
Aletheia Threskeia (a*), Ratna Sofaria Munir (b), Agung Dwi Wahyu Widodo (b),
a) Post Graduate School, Universitas Airlangga, Surabaya, Indonesia
*aletheia.threskeia-2018[at]pasca.unair.ac.id
b) Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
Abstract
Background: Enterotoxigenic Escherichia coli (ETEC) has been found as common cause of diarrhea in developing country. According to previous research by Jayakumari and friends in India, the leaf extract of Pisonia grandis has antimicrobial compounds. Therefore, researcher examines the use of Pisonia grandis leaf extract against ETEC. Objective: To determine whether the leaf extract of Pisonia gradis can be used as antimicrobial agent against Escherichia coli and ETEC. Methods: This was a true experimental research, which is done in the laboratory. The bacteria used is Escherichia coli and ETEC. Pisonia grandis leaves extracted with ethanol 70% is divided in eight different concentrations. Antimicrobial activity performed by broth dilution method to determine the Minimum Inhibitory Concentration (MIC). Results: There was turbidity changes at all test tubes up to fifty percent concentration , including the positive control tube, but there was no change in turbidity in the negative control tube. Growth of bacteria colonies was obtained when the tubes were planted in Nutrien agar. Conclusion: It can be concluded from the results that there was no antimicrobial activity in ethanol extract of Pisonia grandis leaves up to fifty percent concentration against Escherichia coli and ETEC.
Keywords: Pisonia grandis, Escherichia coli, Enterotoxigenic Escherichia coli, ethanol extract
Topic: Health and Medicine