ICAST 2019 Conference

The Use Illumina Sequencing Technique in studying the Diversity of Rumen Bacteria of Bali Cattle Fed on a Ration Consisted of Rice Straw and Elephant Grass
Munir1,2, A. Ako3 , S. Syahrir4, A. Natsir4*

1Animal Husbandry Study Program, Faculty of Agriculture, Animal Husbandry and Fisheries,
University of Muhammadiyah Parepare,South Sulawesi, Indonesia (91111)
2Doctoral Program, Agricultural Science Study Program at Hasanuddin University (UNHAS) Makassar, Indonesia (90245)
3Animal Production Department, Faculty of Animal of Science, Hasanuddin University, Makassar, Indonesia (90245)
4Animal Nutrition Department, Faculty of Animal of Science, Hasanuddin University, Makassar, Indonesia (90245)

* Corresponding author: asmuddin_natsir[at]unhas.ac.id


The diversity of rumen bacteria in ruminant animals is highly determined by various factors, including the breed of the animal and the feed consumed. The purpose of this study was to evaluate the diversity of rumen bacteria of bali cattle due to the provision of ration consisted of rice straw and elephant grass as a basal diet. Three adult bali cattle were given ad libitum a ration consisted of rice straw and elephant grass for four weeks before taking the rumen fluid samples. In addition to the basal diet, each animal was also given a concentrate at the rate of 0.5% of the body weight. Rumen fluid samples were analyzed for pH, NH3 and VFA, while the diversity of the rumen bacteria was determined by the Illumina Sequencing technique. The results of the study showed that rumen conditions of the animal were ideal for rumen bacterial growth, indicated by the value of rumen pH, NH3, and VFA of 6.8, 156.7 mg/L, and 114.3mmol/L, respectively. Furthermore, based on SILVA taxonomy, two dominant phyla, i.e. Bacteroidetes and Firmicutes, were successfully identified in this study. At the genus level, the most dominant group identified was Prevotella 1 followed by the Christencenellaceae R-7 group. The analysis also showed the existence of uncultured rumen bacterium and uncultured bacterium with a percentage of less than 5% of the whole population of rumen bacteria. In conclusion, the feed provided for the animal was good enough to support rumen bacterial growth and the Illumina sequencing technique used in this particular study was able to identify the existence of dominant rumen bacteria as well as the uncultured rumen bacterial of bali cattle under such typical feeding condition.

Keywords: Keywords: rumen bacteria, rumen fermentation characteristics, bali cattle, illumina sequencing technique, Silva Taxonomy, typical feeding condition

Topic: Animal Nutrition and feed technology

Link: https://ifory.id/abstract-plain/3U4TENey8thb

Web Format | Corresponding Author (Munir Munir)