Molecular Responses to Bacteremia in Streptozotocin-induced Diabetes Rats
Edwin Widodo (1*), Gian Suryanatha Hartawan (2), Indah Amalia Amri (2)
1) Department of Physiology, Faculty of Medicine, Brawijaya University
*edwin[at]ub.ac.id
2) Department of Microbiology, Faculty of Veterinary Sciences, Brawijaya University
Abstract
Diabetes mellitus is a metabolic disorder that is characterized by chronic hyperglycemia, which is accompanied by damage, dysfunction of some organs and disturbance of the metabolism. In diabetes type II, there is a decrease of the ability of insulin to work in peripheral tissues (insulin resistance) and β cell dysfunction. As a result, the pancreas is unable to produce enough insulin to compensate for insulin resistance. Infection of Staphylococcus aureus in diabetes mellitus may worsen the condition. It can lead to bacteremia as well as increase the occurrence of inflammation. This research was aimed to know the molecular responses of rats (Rattus norvegicus) treated with streptozotocin and S.aureus via CD4 T cells and TGF-β levels. This research was Completely Randomized Design (RAL) experiment, with five groups of treatment. Group 1 was normal rats, Group 2 was bacteremia treatment with infection of S.aureus 10^8 CFU/mL, and three other groups were 45 mg/kg streptozotocin-induced diabetes with different level of S. aureus infection (10^5, 10^6 and 10^7 CFU/mL). Injections of streptozotocin were done intraperitoneally in high single dose (45 mg/kg). Blood glucose levels was measured after 2 days of STZ injection followed by Staphylococcus aureus injection intraperitoneally. The relative levels of CD4 T cells and TGF-β was measured by flowcytometry. The data was analyzed using One Way Analysis of Variance (ANOVA) (α = 0.05). The results showed an average CD4 levels were relatively experienced 6.9%, 7.9% and 3.5% increase in group 3, 4 and 5 respectively. The average relative levels of TGF-β were decreased 0.7%, 3.1% and 5.1% in group 3, 4 and 5 respectively. In conclusion, the relative levels of CD4 increased while the relative levels of TGF-β decreased in streptozotocin-induced diabetes rats infected with Staphylococcus aureus.
Keywords: CD4, TGF-β, Diabetes mellitus, Staphylococcus aureus
Topic: Biomedical Science