Antibacterial Activities of Curcuma mangga Val. Extract in some Solvents to Staphylococcus aureus and Escherichia coli
Bayyinatul Muchtaromah1, a), Evika Sandi Safitri1, b), Prilya Dewi Fitriasari1, c), Jujuk Istiwandhani1, d)
1 Department of Biology, Faculty of Science and Technology, The State Islamic University of Maulana Malik Ibrahim Malang, Jl Gajayana 50, Malang, Indonesia
Abstract
Curcuma mangga is one of the traditional medicine that has the effect of dealing with infertility problems. Staphylococcus aureus and Escherichia coli are bacteria that is often found as the cause of the female reproductive tract infection. The purpose of this study was to determine the antibacterial activity of C. mangga extract against S.aureus and E. coli in several solvents. C. mangga rhizome was extracted by a maceration method using ethanol (polar), chloroform (semi-polar), and n-hexane (non-polar) solvents. The antibacterial activity test against S.aureus and E. coli using Kirby Bauer method with 100% concentration and microdilution method with concentration (%) 50, 25,12.5, 6.25, 3.13, 1.56, 0.78, 0.39. The highest inhibition zone diameter for S.aureus was obtained by ethanol (10.11 m), chloroform (9.21 mm), n-hexane (6.05 mm) extract while for E. coli respectively achieved by ethanol extract (8.06 mm), n-hexane (5.88 mm) and, chloroform (4.19 mm). The MIC value of ethanol extract to S.aureus was found at concentrations of 3.13% and MBC at 6.25%. The MIC value of ethanol extract on E. coli was obtained at concentrations of 6.25% and MBC at 12.50%. Ethanol extract of C. mangga rhizome produced the most antibacterial activity than chloroform and n-hexane.
Keywords: antibacterial, chloroform, Curcuma mangga, Escherichia coli, ethanol, n-hexane, Staphylococcus aureus
Topic: Microbiology