IMMUNOSTIMULANT ACTIVITY OF TEMULAWAK (Curcuma xanthorrhiza) STARCH TOWARDS SPRAGUE DAWLEY RATS INDUCED WITH CYCLOPHOSPHAMIDE
Tiya Novlita Renggani, Idah Rosidah, Sri Ningsih, Kurnia Agustini
Laboratory of Pharmacology, Center of Pharmaceutical and Medical Technology, Agency for the Assessment and Application of Technology
Abstract
Cyclophosphamide is a chemotherapeutic agent which has immunosuppressant effect against the host immune system, so adjuvant which has immunostimulant effect is often needed, one of the example is the polysaccharide fraction contained in plant starch. Temulawak is a plant that is widely known to have immunostimulant effects, but its starch has not been widely studied. The purpose of this study was to determine the immunostimulant effects of temulawak starch in Sprague Dawley male rats which were induced by cyclophosphamide in vivo. Test animals were divided into 6 groups, namely normal control, cyclophosphamide control, levamisol as a known chemical, and temulawak starch in dose of 200, 400 and 800 mg/ gBW. For 56 days, the test animals are treated with test compounds. Cyclophosphamide induction of 1000 mg/kgBW was given i.p on days 16 and 18. Evaluation of the test animal model was seen from the number of leukocytes and platelets, while the evaluation of immunostimulant activity was seen from the parameters of rat body weight, spleen relative weight, and nitric oxide secretion. The results showed that there were no significant differences in the parameters of body weight and relative spleen weight, but there was an increase in the secretion of nitric oxide (NO) in the temulawak starch dose group of 200 mg / kg compared with the other groups. This increase in NO secretion is associated with an increase in non-specific immune response.
Keywords: immunostimulant activity, cyclophosphamide, temulawak starch, nitrit oxide
Topic: Farmakologi dan Klinik TOOT