Isolation and Antioxidant Activity of ethyl-p-methoxy-cinnamate Compounds from n-hexane Extract Kaempferia galanga L.
Fajar Fauzi Abdullah (a*), Astri Senania (a), Nenden Fauziah(a), Ruchiyat (a), Mohd. Azlan Nafiah (b)
a. Departement of Chemistry, Universitas Garut, Jl. Raya Samarang No.52A Garut, Indonesia.
*fajarfauzi[at]uniga.ac.id
b. Sultan Idris University of Education, Faculty of Mathematics and Sciences, Department of Chemistry, 35900 tg. Malim, perak, Malaysia.
Abstract
Objective: This study was to determine the antioxidant activity of marker compounds contained in Kaempferia galanga L. n-hexane extract, by isolating compounds from Kaempferia galanga L. n-hexane extract and examining antioxidant activity. Method: Kaempferia galanga L. dry powder was macerated with n-hexane for 3x24 hours. n-hexane extract was obtained and evaporated until the solution became 30mL then let it sit on the icebath, the residue obtained was then recrystallized with ethanol. The colorless crystalline solids obtained were then tested for antioxidant activity with the DPPH (1,1-diphenyl-2-pikrilhidrazil) method. Results: Spectroscopic analysis including UV Vis, IR, 1D-NMR and 2D-NMR showed that isolates were phenylpropanoid type compounds derived from hydroxycinamic acid in the form of ethyl-p-methoxy-cinnamic and strong intensity antioxidant activity indicated by IC50 value of 58,851 ppm. The benefits of this study can provide a basic knowledge of the utilization of the potential content of the main compounds from Kaempferia galanga L. for the pharmaceutical preparation field.
Keywords: Kaempferia galanga; Antioxidant Activity; Isolation secondary metabolites; ethyl-p-methoxy-cinnamate
Topic: Chemistry