Malondialdehyde (MDA) of Spermatozoa Levels in Mice (Mus musculus albinus) with Giving Extracts of Water Seed and Neem Leaves (Azadirachta indica A. Juss)
Elsa Lisanti1, Dondin Sajuthi2, Muhammad Agil3, R Iis Arifiantini3, Adi Winarto3, Arwin4
1 Universitas Negeri Jakarta (UNJ) Department of Biology, Faculty of Mathematics and Natural Sciences, Kampus A, Gedung Hasjim Asjarie Lt. 9. Jl. Rawamangun Muka Jakarta Timur, 13220, Indonesia
2 Department of Veterinary Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine, Institut Pertanian Bogor (IPB), Dramaga Bogor 16680, Indonesia)
E-mail : sajuthi[at]indo.net.di
3 Departement Anatomy, Physiology, and Pharmacology,Faculty of Veterinary Medicine, Institut Pertanian Bogor (IPB), Dramaga Bogor 16680, Indonesia
4 Center Application Isotopes and Radiation (CAIR), National Nuclear Energy Agency of Indonesia, Jalan Lebak Bulus Raya No. 49 South Jakarta, Indonesia
Abstract
Neem is a herbal plant that can be used as male contraceptive, by preventing the penetration of spermatozoa in the process of in vitro fertilization. However, the mineral content found in the seeds and leaves of neem can induce oxidative stress in mice, characterized by increased lipid peroxidation potential in the spermatozoa. This study aimed to evaluate the malondialdehyde levels of spermatozoa were epidydimal in mice. To calculate MDA levels in spermatozoa a standard thiobarbituric acid (TBA) method is used. The first group was plot as a control. The four groups were orally treated by neem leaves (two groups) and seeds extract (two groups) in 0.5 mL/mouse/day at doses 0.25 and 0.50 mg/kg bw each for 36 days then were sacrificed. The other four groups were treated same as those in the previous four groups and sacrificed at 36 days post last treatment. Data were analyzed by randomized completely design (RCD) in the test differ of 5%. The results showed that the administration of seed and leaves of water extract of neem did not reduce the MDA levels of sperm mice. The conclusion that the mineral content of neem extracts (seeds and leaves) are safe to use.
Keywords: Seeds, leaves, MDA, mice, neem, spermatozoa
Topic: Biology