BLACK CUMIN SEEDS INCREASE LYMPHOCYTE ACTIVITY IN IFN-Y SECRETION IN SPRAGUE DAWLEY MICE (SD) INDUCED BY DIMETHYLBENZANTRACENE
Titiek Hidayati, Akrom2, Indrayanti3, Sagiran4
1.Public health and Family medicine, Medicine and Health Science Faculty, UMY
2.Pharmacology and Clinical Pharmacy, Pharmacy Faculty, UAD; Ahmad Dahlan Drug Information and Crisis Center;
3.Pathology anatomy department, Medicine and Health science Faculty, UMY
4.Surgeon department, Medicine and health science Faculty, UMY
Abstract
Interferon-gamma (IFN-γ) is one of the central cytokines in the anti-carcinogenesis immune response. Lymphocytes are one of the IFN-y producing cells. Black cumin seeds (BCS) have an active content of thymoquinone and unsaturated fatty acids with biological activity as immunomodulators. This study aimed to determine the effect of administration of BCS extract on IFN-y secretion activity by SD rat lymphocytes induced by dimethylbenzanthracene (DMBA). In vivo experimental study on DMBA-induced SD rats. BCS extract was given with three doses for two weeks before being induced and five weeks during DMBA induction. IFN-y levels in lymphocyte culture supernatants were determined by the ELISA method. The difference in IFN-y levels between groups was analyzed by ANOVA test, the significance of 95%. The results showed that administration of BJH extract for 14 days did not affect cellular composition toward the edge of the test animal. BCS extract can increase IFN-y secretion activity by DMBA-induced SD rat lymphocytes.
Keywords: black cumin seed, IFN-y; DMBA: immunomodulator, carcinogenesis.
Topic: International Symposium of Engineering, Technology, and Health Sciences