Primary Antibody of Aeromonas salmonicida in Tilapia (Oreochromis niloticus) for Detecting Furunculosis
Kurniasih
Gadjah Mada University
Abstract
Tilapia aquaculture is well developed in Indonesia, however, the main problem which may cause loss is bacterial infection, mainly Aeromonas salmonicida which may result in furunculosis. Identification of furunculosis takes a long time and it is often confused by A. hydrophila infection. The aim of study was to produce primary antibody from local isolate of A. salmonicida, for detecting furunculosis based on immunohistochemistry staining. Local isolate of A. salmonicida was attenuated using formaldehyde to obtain the O antigen and further intraperitoneally injected to the rabbits with weekly increased dosage which were 0.5, 1, 2, and 3 mL. The immunization was done in 4 weeks, and at the 5th week the blood sample was collected to make the primary antibody for immunohistochemistry procedure. The result showed that the antigen of A. salmonicida was found in the liver, kidney, spleen, gill, and intestine since the third until tenth day post infection. The reaction of antigen and antibody appeared as brown colour within the cytoplasm of those organs. The conclusion is the primary antibody of A. salmonicida is very sensitive for immunohistochemistry.
Keywords: Aeromonas salmonicida; immunohistochemistry; tilapia; primary antibody
Topic: Agricultural structures and environmental engineering