Phycocyanin from Spirulina plantesis: In silico approach, in vitro evaluation, and storage stability as antidiabetic candidate
Heli Siti Halimatul Munawaroh, Gun Gun Gumilar, Fina Nurjanah, Dewi Kurnia, Ihsanawati, Galuh Yuliani, Asri Peni Wulandari, Isman Kurniawan, Andriati Ningrum

UPI

Abstract

Phycocyanin is the main pigment in Spirulina platensis which is reported has potential to be used as a natural inhibitor for α-amylase and α-glucosidase. The pigment can effectively treat type-2 diabetes mellitus by lowering blood glucose. However, molecular interactions between phycocyanin and the two enzymes are less studied. In this study, an in silico study through molecular docking simulation was carried out to predict the molecular interactions of phycocyanin and both of α-amylase and α-glucosidase. In vitro evaluation of phycocyanin as an anti-diabetic is determined through the inhibitory activity of phycocyanin against α-amylase derived from human saliva. Storage stability in three conditions was evaluated in its application as a functional supplement. Docking simulations between phycocyanin and acarbose as commercial drugs with α-amylase and α-glucosidase enzymes were performed using the AutoDock Tools program. Determination of inhibitory activity was carried out using the FUWA method, while the measurement of absorption values with a UV-Vis spectrophotometer. Storage stability study was performed on solid and solutions-stated of phycocyanin at three different conditions, namely dark conditions at -4°C, dark conditions at room temperature, and bright conditions at room temperature. The stability was determined by measuring the absorption values at λ620 and λ652 using UV-Vis spectrophotometer. Molecular docking simulations show that phycocyanin, inhibits by binding to the active site of the enzyme, causing disruption of the attachment of the substrate to the enzyme. The simulation indicates that phycocyanin has potency to be used as a candidate for anti-diabetic natural therapy agents. An in vitro inhibition activity test showed that phycocyanin inhibits α-amylase from human saliva at average 50.13%. Storage stability tests showed that the concentration of phycocyanin in the form of solutions and solids, was stable for seven days of testing under conditions of dark storage and low temperatures.

Keywords: Diabetes mellitus type-2, Phycocyanin, Molecular Docking, Stability, α-amylase, α-glucosidase

Topic: Chemistry

Link: https://ifory.id/abstract-plain/BjcEZ4LXnm8U

Web Format | Corresponding Author (Heli Siti Halimatul Munawaroh)