Chemopreventive Activity of Roselles Hexane Fraction against Breast Cancer by In-Vitro and In-Silico Study (On-going research)
Nabila Kaulika (a*), Rifki Febriansah (a)
a) Department of Pharmacy, Faculty of Medical and Health Science, Universitas Muhammadiyah Yogyakarta
Brawijaya Street, Tamantirto, Kasihan, Bantul, Yogyakarta 55183, Indonesia
*nabilakaulika[at]gmail.com
Abstract
Breast cancer is ranked the highest percentage of the new cases and deaths in women around the world. Cancer treatments that currently exist cause side effects that are detrimental to patients, thus triggering the development of cancer treatment that comes from nature or herbal plants. Rosella (Hibiscus sabdariffa L.) is a plant that is known contains flavonoids which are potential as anticancer agent. This research method was experimental study. This study to analize the Roselle n-hexane fraction as a chemopreventive agent, with an antioxidant test using the DPPH method, in-vitro cytotoxic test of T47D cancer cells line by MTT method, and in-silico test using Vina molecular docking method. The results of the antioxidant test showed the IC50 value of 4259 ug/mL. Cytotoxic activity of the fraction on T47D breast cancer cells showed IC50 values of 331.86 ug/mL. Molecular docking results obtained a docking score that shows the binding energy of cyanidin 3-O-glucoside to EGFR and HER-2 proteins of -8.2 kcal/mol and 8.1 kcal/mol, respectively. Based on these results, it can be concluded that the Roselle n-hexane fraction has a potential as a chemopreventive agent based on molecular docking and cytotoxic activity against T47D breast cancer cells.
Keywords: Hibiscus sabdariffa; cytotoxic MTT; antioxidant DPPH; molecular docking; T47D breast cancer
Topic: International Symposium of Engineering, Technology, and Health Sciences