Combination of Coconut Water and Banana Extract on New Dogashima Medium Enhance Multiplication of Vanda tricolor
Innaka Ageng Rineksane (a*), Arum Wahyu Ningsih (a), Agung Astuti (a) and Gatot Supangkat (a)
a) Department of Agrotechnology, Faculty of Agriculture, Universitas Muhammadiyah Yogyakarta
Jalan Brawijaya, Kasihan, Bantul, Yogyakarta 55183
*rineksane[at]gmail.com
Abstract
Vanda tricolor is an endemic orchid on the slopes of Mount Merapi whose existence is threatened with extinction due to eruption and exploitation of its natural habitat. The rapid propagation of Vanda tricolor can be done through in vitro multiplication. Multiplication can double the number of shoots by adding growth regulators in the medium. Substitution of synthetic growth regulators with organic material for multiplication can reduce the cost of propagation of Vanda tricolor. This study aims to obtain the best organic extract for multiplication of Vanda tricolor. This research was compiled using a randomized completely design single factor. The treatments tested were New Dogashima Medium supplemented with 0.5 mg L-1 thidiazuron, coconut water 150 ml L-1, coconut water 150 ml L-1 + banana extract 150 g L-1 and coconut water 150 ml L-1 + tomato extract 150 ml L-1. Each treatment was repeated 5 times, each replication consisted of 3 samples. The results showed that the treatment of New Dogashima Medium supplemented with coconut water 150 ml L-1 + banana extract 150 g L-1 could replace the role of synthetic growth regulators in the multiplication of Vanda tricolor orchids as indicated by the parameters of the growth time of shoots, number of shoots and number of leaves.
Keywords: Orchid, Medium, Organic extract, In vitro, Substitution
Topic: Crop and Crop Management