The Effect of Frequency of Dressing Replacement with Betaine Polyhexanide 0.1% on the Formation of Collagen and Epithelial Tissue in Albino Wistar Rats with Grade IIA Burns
Heri Kristianto(1*), Obed Trinurcahyo Kinantyo Paundralingga(2), Danik Agustin Purwantiningrum(2), Lu-luil Maknun(1), Rizky Oktavia Primasari(1), Agung Waluyo(3)
1) School of Nursing, Medical Faculty, Universitas Brawijaya
2) Anatomy and Histology Laboratory, Medical Faculty, Universitas Brawijaya
3) Faculty of Nursing, Universitas Indonesia
Abstract
Background: Moist wound healing is a method of retaining moisture so that migration of epithelial cells and synthesis of collagen can increase. One of the liquids that can be used is Betaine Polyhexanide 0,1%. Purpose: This study aimed to determine the effect of frequency of dressing replacement with Betaine Polyhexanide 0.1% on the formation of collagen and epithelial tissue in albino wistar rats with grade II A burns. Methods: This study used Posttest Only Randomized Controlled Group Design conducted on 25 male rats within a period of 13 days divided into 5 groups. Each group comprised 5 rats; those are “every 12 hour” group, “every 24 hour” group , “every 36 hour” group, “every 48 hour” group and “control” group. The variable measured in this study is the percentage of epithelial and collagen. Results: The Shapiro-Wilk/Kolomogorov Smirnov and Levene test have a significant value of (p > 0,05) indicating normal distribution and homogeneity. One Way ANOVA test revealed a significant difference in the synthesis of collagen with p-value of 0.002 (p<0.05) but there was no significant difference in the formation of ephitelial with p-value of 0.561 (p > 0.05). The highest score was found in Betaine Polyhexanide 0,1% in the every 24 hour group and the second is in the 48 hour group for collagen and control group for ephitelial. Conclusion: It can be concluded that the treatment of burns grade II A with Betaine Polyhexanide 0.1% every 24 hours can optimize the formation of collagen and ephitelial tissue.
Keywords: betaine polyhexanide, collagen, epithelial tissue, burns, frequency of dressing replacement
Topic: Nursing