Effect of Sucrose Addition in PDA (Potato Dextrose Agar) Media to The Growth of Mold and Yeast
Anas Fadli Wijaya, Dwi Yuliandini
Graduate Student of Immunology, Postgraduate School, Universitas Airlangga, Surabaya, Indonesia
Medical Laboratory Techniques,Polytechnic of Health, Mataram, Indonesia
Abstract
Fungi are heterotrophic organisms which require organic compound as their source of nutrition. Based on the cell type, fungi are classified into unicellular (yeast) and multicellular (mold). One of standard laboratory examination for fungi is microbiological culture using PDA (Potato Dextrose Agar) media. The addition of sucrose in the PDA is to enrich the media composition and the energy source that will aid the growth process of mold and yeast. This study aimed to determine the effect of sucrose addition in PDA media to the growth of mold and yeast using quasi-experimental design. Sample of mold and yeast used in this research were Rhizopus sp and Candida albicans, respectively. Candida albicans isolates were made into suspension adjusted to be equivalent with the turbidity of 0,5 Mac Farland and diluted 106 times. Growth diameter of Rhizopus sp was measured and the colony number of Candida albicans were counted on PDA supplemented with 1 g, 2g, and 3g, respectively. The results were compared to those of control group and analyzed quantitatively. One Way Anova test showed p value of 0.000 (p<α) on Rhizopus sp and Kruskal Wallis test showed p value of 0.009 (p>α) on Candida albicans, suggesting a significant effect of sucrose addition to the growth of mold but no effect to the growth of yeast.
Keywords: Sucrose, PDA media, Candida albicans, Rhizopus sp, Mold, Yeast
Topic: Health and Medicine