Molecular Screening of Three Type 2 Diabetes Mellitus Families Using RAPD-PCR (Random Amplified Polymorphic DNA)
Miftahul Mushlih1,2, Tri Rachmawati Ningsih1, Evi Rinata2, Rohman Dijaya3, Andika Alviameyta3, Puspitasari1, Andika Aliviameita1 Siti Cholifah1, Dian Delta2 & Dwi Anggorowati Rahayu2,4,
1) Faculty of Health Science, Universitas Muhammadiyah Sidoarjo,
Jl. Mojopahit No. 666, Sidoarjo, Jawa Timur.
2) Indonesian Genetic and Biodiversity Community, Jl. Ikan Mujair No. 15, Tunjung Sekar, Lowok Waru, Malang.
3) Faculty of Science and Technology, Universitas Muhammadiyah Sidoarjo,
Jl. Mojopahit No. 666, Sidoarjo, Jawa Timur.
4) Department of Biology faculty of Mathematic and Natural Science, Universitas Negeri Surabaya, East Java Indonesia.
Abstract
Type 2 Diabetes Mellitus (T2DM) is disorder caused by the loss of cell- β insulin secretion or insulin resistance. T2DM occurs by genetic and environmental factor. This study was conducted by analyze the inheritance of polymorphism in T2DM families. The method was used is RAPD-PCR (Random Amplified Polymorphic DNA) using F4 5--GTCTTGGGCA3- primer. Three families whose had a history of type 2 diabetes mellitus used. They are composed by 4 samples as positive control (diagnosed with diabetes mellitus), and 9 samples allele analyzed. 2 sampel from other sources was used as negative control. For the results, there were 7 of 9 samples might positive allele of T2DM.
Keywords: Type-2 Diabetes Mellitus, Polymorphism, RAPD-PCR, Pedigree
Topic: Biomedical Science