Occurence of Clavibacter michiganensis subsp. sepedonicus on Potato in South Sulawesi
Baharuddin (a,c*), A. Pabborong (b), Tutik Kuswinanti (a,c), Untung Surapati (a), Mustika Tuwo (c,d)
a) Departement of Plant Pests and Diseases, Faculty of Agriculture, Hasanuddin University, Makassar, South Sulawesi, 90245
*baharuddin[at]agri.unhas.ac.id
b) Indonesian Agriculture Quarantine, Makassar Branch Office, Makassar, South Sulawesi, 90245
c) Agricultural Biotechnology Laboratory, Faculty of Agriculture, Hasanuddin University, Makassar, South Sulawesi, 90245
d) Departement of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar, South Sulawesi, 90245
Abstract
Bacterial ring rot caused by a gram-positive Coryneform bacterium C.m subsp. sepedonicus is an important disease in potato crops in the world. The disease is until now still belong to an A1 quarantine pathogen in Indonesia, although it was found in West Java since 2013. The objective of this study was to know the presence of bacterial ring rot in four potato district areas in South Sulawesi. Diseased samples were conducted from potato fields and storage warehouses in Enrekang, Gowa, Jeneponto and Bantaeng. Potato tuber samples were cutted and observed their vasiculer vessels and then isolated and grown the bacteria on NA and NBY media. Bacterial isolates were morphological and physiological characterized as well as patogenicity on eggplant and PCR test using specific primer for Cms 50F and Cms 50 R. The results showed that Cms has become widespread in four districs in South Sulawesi. The disease incidence of bacterial ringrot in these districs reached above 30 %. All of 14 isolates were obtained from the results of a standard methods for identification (EPPO, 2006) showed that the presence of DNA band size of 224 bp, which indicated positively belong to C.michiganensis subsp. sepedonicus.
Keywords: bacterial ring rot, PCR.
Topic: Other Agricultural and biosystems topic