ACTIVITIES TEST FOR THE REDUCTION OF OBESITY INDEX FROM ROBUSTA GREEN COFFEE SEED EXTRACT (Coffea canephora) TO MALE WISTAR STRAIN WHITE MOUSE
Seno Aulia Ardiansyah (a), Anggi Restiasari (b), Ditta Restiany Noer Utami (c)
(a) Laboratorium Farmakologi, Jurusan Farmasi, Departemen Farmasi, Sekolah Tinggi Farmasi Indonesia, Kota Bandung
(b) Laboratorium Farmakologi, Jurusan Farmasi, Departemen Farmasi, Sekolah Tinggi Farmasi Indonesia, Kota Bandung
(c)Laboratorium Farmakologi, Jurusan Farmasi, Departemen Farmasi, Sekolah Tinggi Farmasi Indonesia, Kota Bandung
Abstract
Obesity is one of the problems in the community. The consumption of green coffee beans robusta (Coffea canephora) is one of the alternatives used for weight loss, especially in green coffee beans robusta containing higher secondary metabolite compounds such as chlorogenic acid including a group of phenol compounds that reduce the obesity index, and as antioxidants. The aim of this study was to find out that, the reduction of obesity index to male wistar strain white mouse given by ethanol extract of green coffee beans for 24 days against obesity model which induced by diet high fat diet for 50 days. Green coffee beans robusta extraction using maceration method durimg 3x24 hours using solvent ethanol 96%. The treatment group consisted of negative group was given CMC 1%, positive control group was given orlistat 30 mg/kg BW, dose group ethanol extract ethanol of green coffee beans robusta including dose 200 mg/kg BW, 400 mg/kg BW, and dose 600 mg/kg BW. Ethanol extracts of green coffee beans robusta at a dose of 200 mg/kg, 400 mg/kg, and 600 mg/kg had its effectiveness as a weight loss percentage value of 8.11%, 12.32% and 10.79%. A dose of 200 mg/kg, 400 mg/kg BW and 600 mg/kg BW can lower the value of the index parameter lee as the obesity index with the value of the percentage decrease of 2.94%, 3.88% and 3.78%. Based on statistical analysis with ANOVA on weight loss shows the value of significance (P < 0,05) was found on a dose of 400 mg/kg BW and 600 mg/kg BW.
Keywords: Obesity, Green Coffee Beans Robusta, Chlorogenic Acid, Weight loss, Index lee.
Topic: Farmakologi dan Klinik TOOT