Application Of RAPD Markers to Analyze Genetic Diversity Among Date Palm (Phoenix dactylifera L.) Genotypes
Annisa Fadhilah Sitepu (a), Eva Sartini Bayu (a*), Luthfi Aziz Mahmud Siregar (a), Lollie Agustina P. Putri (a), Asni (b)
(a) Faculty of Agriculture, University of Sumatera Utara, Jalan Prof. A. Sofyan no.3 Kampus USU Padang Bulan, Medan 20155 Indonesia
*Corresponding author:email evagirsang61[at]gmail.com
(b)Laboratory staff of biotechnology laboratory
Abstract
Date palm cultivation can be done generatively and vegetatively. Tissue culture is one of the techniques used for vegetative date palm cultivation. The weakness of this technique is the presence of genetic variations in the results of propagation through tissue culture known as somaclonal variation. One type of molecular markers commonly used for variations in genetic variation is RAPD (Random Polymorphic Amplified DNA) markers. The aim of the research was to analyze genetic variation of Date Palm genotypes using three RAPD primers - OPD-13, OPD-20, and OPO-16. This research is conducted by taking 8 genotypes from tissue cultur propagation and 2 genotypes from seed propagation. Calculation of distance matrix or genetic inequality is done by two types of descriptive analysis of diversity such as Principal Coordinates Analysis (PcoA) and Neighbor-Joining Tree (NJtree) using DARwin 6.0 software. Based on the cluster analysis, 10 samples of date palm group into three major groups with a total of molecular diversity was 83.11%, The range of dissimilarity coefficient was 0 – 0.6.
Keywords: date palm, genetic diversity, RAPD marker
Topic: International Conference on Sustainable Agriculture