Celery (Apium Graveolens L.) Extraction as the Inhibition of Pathogenic Microorganisms in Broilers
Yuli Frita Nuningtyas, Halim Natsir, Osfar Sjofjan, Irfan H.Djunaidi
Lecturer Animal Nutrition, University of Brawijaya, Malang, Indonesia
Abstract
The aims of the research was to determine the ability of the inhibition bioactive compounds on celery as a pathogenic bacteria such as Salmonella, Escherichia coli and Clostidium perfrinens were found in broiler digestive tracts. The stages of the study were the determinated of celery plants, phytochemical content test in celery extraction and inhibitory test on Salmonella, Escherichia coli and Clostidium perfrinens with P0 concentration: negative control (aquades), P1: positive control (5 µg / ml amoxicilin), P2: 2,5 mg / ml celery extraction, P3: 5 mg / ml celery extract, and P4: 10 mg / ml celery extract. The variables observed were the inhibition zone diameter using the disc method. The data obtained is tabulated with the Microsoft Excel program, then analyzed using a completely randomized design (CRD) where the significantly differences will be analyzed using Duncans analysis. Based on the results study showed that the results of plant determination were celery (Apium gravolens, Linn). The addition of celery extract with a concentration of 10 mg / ml showed a higher inhibitory power compared to the use of antibiotics against salmonella and E coli bacteria.
Keywords: Clostridium perfringens, extraction, Escherichia coli, Salmonella, celery
Topic: Animal Nutrition, Animal Production, and Veterinary Science