Profile of Oil Palm (Elaeis Guineensis Jacq.) DNA Band DxP 540 NG Variety Based on RAPD (Random Amplified Polimorphism DNA) Marker
Rumata Yohana Diantoro (a), Eva Sartini Bayu (a*), Diana Sofia Hanafiah (a), Lollie A.P. Putri (a), Asni (b)
a) Agroteknologi Departement, Faculty of Agriculture, Universitas Sumatera Utara, Jl. Prof. A. Sofyan No. 3, Medan 20155, Indonesia
*evagirsang61[at]gmail.com
b) Laboratory Plant Biotechnology Faculty of Agriculture, Universitas Sumatera Utara,
Jl. Prof. A. Sofyan No. 3, Medan 20155, Indonesia
Abstract
RAPD marker is an informative molecular marker, could enhance the efficiency of early selection stage and reduce selection time in oil palm breeding. The aim of this study was to look at the percentage of polymorphic bands of genetic diversity in the DxP 540 NG Variety based on three random amplified polimorphism DNA marker. A total of 20 oil palm leaf samples of the DxP 540 NG variety were isolated. The size of the bands ranged from 446-1574 bp measured using UVITEC Cambridge FireReader software. The absorbance values of DNA stocks at A260 / 280 ranged from 1.37-2.50. The DNA concentration (μg / ml) ranged from 95.1-1036. The total banding pattern produced 14 bands with 100% polymorphic bands.
Keywords: oil palm, polymorphism, RAPD.
Topic: International Conference on Sustainable Agriculture