In-Vitro Investigation of Cytotoxicity of West Java Curcuma longa and Potential Therapeutic use against Breast Cancer
Azzakiyah. Fatimah & Azzahra. Anggraeni , Triananda. Firda, Yuniarti, Lelly
1) Faculty of Medicine, Universitas Islam Bandung
2) Faculty of Mathematics and Natural Sciences, Universitas Islam Bandung
3) Medical and Molecular Biology, Faculty of Medicine, Universitas Islam Bandung
Abstract
Breast cancer is second leading cause of death for women and in Indonesia can be increase incidence each year. Previous studies has found the used of Curcuma longa as antioxidant, antidiabetic, antiviral even antiangiogenic. The role as antioxidant and antiangiogenic give Curcuma longa as one of potential plant for therapy of cancer. This study aim to know potential therapeutic use of this plant originated from West Java for breast cancer and know the cytotoxic range. Curcuma longa prepared as ethanol extraction using reflux methods. In-vitro investigation used MCF7 cell line. We conduct the cytotoxic examination using MTT assay of the extract to the cell line. Another examination is secondary for secondary metabolites. The screening results give positive for flavonoid and tannin as secondary metabolites and the IC50 from MTT assay of MCF7 is 0.256µg/ml (95% CI 0-3.04µmol). The ethanolic extract turmeric rhizome had high anticancer activity with IC50 values of 0.256µg/ml, revealing that Curcuma longa inhibits MCF7 proliferation. This result shows the potential of Curcuma longa for further research as therapeutic agent for breast cancer.
Keywords: Curcuma longa, breast cancer, therapy of cancer
Topic: Drug Discoveries and Development