In Vitro Propagation of Pineapple (Ananas comosus) Shoots from Sipahutar North Sumatera Indonesia
Yuliana Fernando(1), Fauziyah Harahap(2*), Dikky S. Diningrat(2), Rosmayati(3)
(1)Biology Education of Postgraduate School Universitas Negeri Medan, Jalan Willem Iskandar Pasar V Medan Estate, North Sumatera 20221, Indonesia.
(2)Department of Biology, Faculty of Mathematics and Natural Sciences Universitas Negeri Medan, Jalan Willem Iskandar Pasar V Medan Estate, North Sumatera 20221, Indonesia.
*fauziyahharahap[at]gmail.com
(3)Departement of Biology, Faculty of Mathematics and Natural Science, Universitas Sumatera utara, Jalan Bioteknology No.1, North Sumatera 20155, Indonesia
Abstract
Pineapple is a plant that needs to be developed on a plantation scale because the fruit has economic important. The research was conducted to determine the best media combination on the growth of pineapple from sipahutar In Vitro. The research used complete randomized design (CRD) factorial which is consisting of 12 treatments with 3 times repetitions. The parameters observed were number of shoots, leaves and roots. Data was analyzed by using ANOVA, and DMRT as follow up. The results showed addition of BA and vitamins have effect on propagation of pineapple shoots from sipahutar. The highest number of shoots was 8.00 shoots and 19.00 leaves produced MS + Vit 2 ppm + BA 2 ppm media. The highest root number of 3.67 produced MS + Vit 0 ppm + BA 0 ppm media.
Keywords: Benzyl Adenin, in vitro, Pineapple,Vitamin
Topic: Biochemistry, Biotechnology and Pharmacy