Lower Adiponectin Level In Obese People: A Case Control Study Among Javanese
Nia Lukita Ariani (a*), Ahmad Hamim Sadewa (b), Arta Farmawati (b)
a) Department of Nursing Science, Tribhuwana Tunggadewi University, Malang, East Java, Indonesia 65144
*nia.lukita[at]unitri.ac.id
b) Department of Biochemistry, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia 55281
Abstract
Adiponectin has a protective role in metabolic syndromes. Adiponectin gene polymorphism +276G>T (rs1501299) was reported has a strong relationship with increased adiponectin level. However, reports about this variant are still inconsistent in obese people. We investigated the association of adiponectin gene variant with circulating adiponectin. The study was performed with 110 apparently healthy adults about 20-40 years old Javanese people. Pregnant subjects and subjects either under diabetic or other metabolic syndrome medications, were excluded. Plasma was used to measure 8- hours fasting glucose and adiponectin level. PCR-RFLP examined DNA genotyping. The data were analyzed using one-way ANOVA, Fisher Exact test, independent t-test, and Mann Whitney test (p<0.05). Genotyping results showed three genotypes of this polymorphism, namely GG, GT, and TT. No significant difference in genotype distribution related to circulating adiponectin both in obese and lean group. Despite the fact about adiponectin lowering action in the obese group, this polymorphism may neither a risk factor nor protective variant for the Javanese population. These findings assert the adiponectin gene variant still vary in influencing circulating adiponectin among the different population.
Keywords: ADIPOQ gene, circulating adiponectin, metabolic syndromes, obesity, SNP +276G>T (rs1501299)
Topic: Biomedical Science