Increase of TNF-Alpha After Capsaicin Administration
Fery Setiawan (a), Jenny Sunariani (b*), Meyvia Rifka R (c)
a) Postgraduate Student Universitas Airlangga
Jalan Airlangga 4-6, Surabaya 60285, Indonesia
b) Oral Biology Department Faculty of Dentistry Universitas Airlangga
Jalan Prof. Dr. Moestopo 47, Surabaya 60132, Indonesia
*jenny-s[at]fkg.unair.ac.id
c) Graduate Student Faculty of Dentistry Universitas Airlangga
Jalan Mayjend Prof Dr Moestopo 47, Surabaya 60132, Indonesia
Abstract
Wound healing is still remaining a problem worldwide, especially in dentistry. Wound healing has four stages , that in this article will be focused on the inflammatory phase. TNF-alpha is cytokine related with wound healing and one of its receptor called Tumor Necrosis Factor Receptor 1 (TNFR1) related with TrkA and NF-KB. The former relates with peptidergic nociceptors (Substance P (SP), Calcitonine Gene Related Protein (CGRP)) and the latter relates transcription factor. Capsaicin is one of the traditional herbals that has vasodilatation effect, so that it can enlarge the blood vessels and shorten the duration of wound healing. This study was analytic experimental laboratory. Post weaned wistar rats (rattus norvegicus) were divided into three groups, K0 negative control group, K1 positive control group, and K2 treatment group. Both K1 and K2 were administered with Aggregatibacteractinomycetemcommitans serotype b on the surface of gingiva, and K2 was alse administered with extract cayenne pepper with dose 0.0912 mg/kg/day. A week after the treatment, the rats were sacrificed with thionembutal and then it was done HE staining and immunohistochemistry method using polyclonal antibody on TNF-Alpha and TRPV-1 expression. Kolmogorov-smirnov and posthoc test showed that TRPV-1 count and TNF-Alpha expression of the K2 to be significantly higher than the K0 and K1 groups (p<0.05), while there was no significant difference in the TRPV-1 and TNF-Alpha expression between K0 and K1 groups(p>0.05). Capsaicin promotes the number of TNF-Alpha so that it can be confirmed as the alternative treatment of periodontitis.
Keywords: Capsaicin, TRPV-1, TNF-Alpha, TNFR1, TrkA, NF-KB
Topic: Health and Medicine