GENETIC DIVERSITY OF THREE SUNU GROUPERS SPECIES (Plectropomus spp)
Sang Ayu Made Putri Suryani (a), I Wayan Arya (b)

(a) Aquatic Resources management department University of Warmadewa, Denpasar, Bali
Email: suryanip[at]rocketmail.com
(b) Aquatic Resources management department University of Warmadewa, Denpasar, Bali

Abstract

Fish seed quality may be decreased as a result of inbreeding that is a marriage between two individuals who have the same genetic trait. Improved seed quality can be done with the genetic approach to parent selection and crossbreed. Quality seeds which are taken from natural populations are determined by the genetic diversity of a population. Inbreeding can lower the quality of seed because it can increase the number of homozygosity and heterozygosity otherwise decrease the number.This study aims to determine differences in genetic variation three species of grouper groupers (Plectropomus maculatus, Plectropomus leopardus, and Plectropomus oligacanthus) and know the kinship of the three species of grouper. While the benefits of this research can provide information on genetic diversity in natural coral trout grouper, expected inbreeding can be avoided. Muscle and liver tissue was used as a sample for electrophoresis analysis with twelve kinds of enzymes are aspartate aminotransferase (AAT), Alcohol dehydrogenase (ADH), Estrase (EST), -Gliserofosfat dehydrogenase (-GPD), Glukofosfat isomerase (GPI), Isocitrate dehydrogenase (IDH), Lactate dehydrogenase (EST), Malic dehydrogenase (MDH), Malic Enzyme (ME), 6-phosphogluconate dehydrogenase (6- PGD), phosphoglucomutase (PGM), and sarcoplasmic enzymes (SP). Three polymorphic loci in Plectropomus oligacanthus species are 6-PGD, GPI-2, and PGM. In Plectropomus maculatus, and Plectropomus leopardus all monomorphic loci. The three polymorphic loci on Plectropomus oligacanthus meet the Hardy-Weinberg balance with X2 value for 6-PGD value is 0.123 for GPI-2 is 0.028. And PGM is 0.028. Plectropomus oligacanthus has a polymorphism level of 0.188, the total number of the focusing allele is 1,154, and the average heterozygosity is 0.023. The genetic distance between the sepsis population based on twelve enzymes showed between Plectropomus maculatus, and Plectropomus leopardus had a spacing of 0.134, between Plectropomus leopardus and Plectropomus oligacanthus of genetic distance 0.196 and between Plectropomus maculatus and Plectropomus oligacanthus the genetic distance was 0.200. The larger the genetic distance between the species, the kinship relationship will be farther away. The distant kinship is owned by Plectropomus oligacanthus.

Keywords: Genetic diversity, Grouper species, Plectropomus sp,

Topic: Biology

Link: https://ifory.id/abstract-plain/NkEjzn6yPKCL

Web Format | Corresponding Author (Sang Ayu Made Putri Suryani)