Inhibition of Listeria monocytogenes by natural antimicrobial
T Budiati*1, Y Wibisono1, R A Pambayun2, M F Fahrezy2, R Ariyani2, E Kurniawati1, W Suryaningsih1, S O N Yudiastuti1 and A Bakri2
1 Food Engineering Department, Politeknik Negeri Jember, Mastrip PO Box 164
Jember 68101 East Java, Indonesia
2 Food Industrial Technology Department, Politeknik Negeri Jember, Mastrip PO
Box 164 Jember 68101 East Java, Indonesia
Abstract
The aim of the study was to evaluate antimicrobial activity of essential oil from plants as natural antimicrobial to inhibit Listeria monocytogenes. A total 6 essential oil extracted from galangal (Alpinia galanga), ginger (Zingiber officinale), lemongrass (Cymbopogon citratus), orange skin (Citrus sinensis), rosemary (Rosmarinus officinalis) and turmeric (Curcuma longa). By using Minimum Inhibitory Concentration (MIC), the highest and the lowest concentration of essential oil to inhibit the growth of L. monocytogenes was found on turmeric (7.394 % + 0.028%) and lemongrass oil (0.219 % + 0.001 %), respectively. By using disc diffusion assay, the highest and the lowest antimicrobial activity against L. monocytogenes was shown by lemongrass oil (7.460 + 2.792 mm) and orange skin oil (0 + 0 mm), respectively. Out of the essential oil tested, lemongrass oil showed the most promising antibacterial activity inhibit L. monocytogenes.
Keywords: Antimicrobial; Essential oil; Listeria monocytogenes; Natural
Topic: Food Safety