The Blight-Resistance Gene Response to Bacterial Leaf Blight Disease in Isogenic Varieties through Pathogenicity Test
Indra Kurniawan saputra (a), I Made Artika (b), Tasliah (c*)
(a) Department of Biology, Faculty Mathematics and Natural Sciences, State University of Malang, Indonesia
(b) Department of Biochemistry, Faculty of Mathematics and Natural Sciences, IPB University, Indonesia
(c)Molecular Biology Division, Indonesian Center for Agricultural Biotechnology and Genetic Resources Reseacrh and Development (ICABIOGRAD), Indonesia
*tasliah1[at]yahoo.co.id
Abstract
Bacterial Leaf Blight (BLB) disease is a one-factor cause of decreasing rice production in Indonesia. This disease caused by Xanthomonas oryzae pv oryzae (Xoo) which infected rice leaves to yellowish. Xoo isolates were obtained from three different locations namely West Java, West Sumatera, and West Borneo. Each isolate was identified by using primer specifics in colony Polymerase Chain Reaction (PCR) techniques. Isogenic strains that contain resistance genes were infected and observed through pathogenicity tests. Based on results, Code variety holding Xa4-Xa7 gene had a high resistance level on Xoo infection from all locations compared to other isogenic and popular rice strains. In the meantime, IRBB5 holding Xa5 gene survived the attack of Xoo from West Java and West Borneo properly. The infection intensities on Code and IRBB5 consecutively are 1.62% and 3.55% for West Java isolates, 2.10% and 2.97% for West Sumatera Isolates, 2.17% and 4.45% for West Borneo isolates
Keywords: Bacterial Leaf Blight (BLB), Isogenic varieties, Resistance genes
Topic: Biotechnology