Antioxidant Activity of Ethanolic Extract, n-Hexane Fraction, Ethyl Acetate Fraction and Water Fraction of Garut Orange Leaves (Citrus reticulata Blanco)
Faizah Min Fadhlillah, Wiwi Oktaviani and Ria Mariani
Garut University
Abstract
Antioxidants are compounds that are able to eliminate, cleanse, and resist radical effects. Antioxidants can stabilize free radicals by complementing the lack of electrons possessed by free radicals. Several studies have shown that citrus leaves of other spesies have been shown to have antioxidant activity. This study aims to determine the antioxidant activity of ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction of garut orange leaves (Citrus reticulata Blanco) using DPPH (2,2-Diphenyl-1-Picrylhydrazyl) method. Garut orange leaves were extracted with ethanol 96% by macerator apparatus. Concentrated extracts were fractionated by liquid liquid extraction method with n-hexane, ethyl acetate and water solvent. Furthermore, the ethanolic extract, n-hexane fraction, ethyl acetate fraction and water fraction of garut orange leaves were tested for antioxidant activity using the DPPH (2,2-Diphenyl-1-Picrylhydrazyl) method. The antioxidant activity test results showed that ethanolic extract of garut orange leaves had better antioxidant activity, followed by n-hexane fraction, ethyl acetate fraction and water fraction.
Keywords: garut orange leaves, antioxidant activity, DPPH
Topic: Biology