SURVIVAL OF SUGARCANE WHITE GRUB IN SOIL WITH TREATMENT OF ENTHOMOPATHOGENIC FUNGI
Tri Harjaka, Edhi Martono dan Witjaksono
1Departement of Pests and Plant Diseases, Faculty of Agriculture, Universitas Gadjah Mada (UGM), Indonesia. Telph. (Fax): (0274) 523 926,
Abstract
White grub, Lepidiota stigma is one of destructive pest of sugarcane in Indonesia. The fungus Metarhizium anisopliae is known as pathogen of this pest. The research aims to determine the survival of L. stigma larvae on soil treated with the fungus M. anisopliae. Fungi reproduced in the laboratory using sterile corn media for 30 days, then harvested and treated in sandy loam soil with a concentration series 105-108 spores / gram. At each concentration series tested 10 larvae of the second instar of L. stigma with three replications. During the larval rearing used fresh carrots as feed and feed replacement is done each time the same week of observation. The results showed that the fungus M. anisopliae is able to inhibit the development of the second instar larvae of L. stigma to turn to the third instar, pupa and into adulthood. Highest inhibition occurs when third instar larvae phase does not eat, and it ranged between 46-100%, whereas inhibition when entering the third instar stadia ranged between 16-56%. Treatment of M. anisopliae in soil with concentrations of 108 spores / gram gave the highest inhibition reached 100% in third instar larvae of L. stigma, while the treatment of 105, 106 and 107 spores / gram still managed to hold its development of larvae reaching prepupa. Treatment of the fungus at a concentration of 105 and 106 spores / gram is capable of causing mortality of L. stigma larvae reached 93.33% and 96.67%, while the treatment of 107-108 spores / gram already caused 100% larvae failing to reach adult life.
Keywords: sugarcane white grub, survival, insect pathogens, soil treatment
Topic: Crop and Crop Management