Porosity Examination of Demineralized Dentin Material Membrane as Guided Bone Regeneration
Pratiwi Soesilawati (a*), Rezka Ajeng Pradhitta (a)
*Oral Biology Department of Faculty of Dentistry, Airlangga University
Undergraduated Student of Faculty of Dentistry, Airlangga University
Abstract
Bone is one of the bodys part which can repair from damage. Bone damages can be caused by trauma, tumors, infections, congenital defects, and also due to surgical procedures. If not treated well, alveolar bone will lose its volume by 40% -60% within 3 year, and the 10% of bone regeneration due to fracture or trauma experiences delays or failure to union. GBR is a technique using a barrier membrane that is placed into the bone defect to provide bone cells with space intended for bone regeneration to increase bone formation which is grows slower than fibrous tissue The pore size of the barrier membrane is very important to prevent excessive fibrous tissue into the defects site and to facilitate neovascularization and bone formation. Objective: This study aims to determine the porosity of DDMM. Method: Bovine dentin is carried out by the process of demineralization and freeze drying until the enamel tissue dissolves. Dentine is cut to a size of 5x5 mm with a thickness of 0.5 mm. Perform double packaging and sterilization using gamma irradiation rays. Membrane porosity seen through SEM examination. Gold coating is done to remove the weak conductivity from the sample before examinating. Samples are put in the sample room at a voltage of 20kV and an magnification of 300. The image is obtained by detecting a secondary signal emitted of sample by an electron beam which produces a CRT (Catode Ray Tube) screen image. SEM micrographs are converted into binary images and further analyzed by Image J software. Conclusions: The average porosity size of the samples seen is 300-500 μm. This porosity size is ideal for GBR membranes that can increase cell proliferation and aggregation to defect sites, as well as good permeability, neovascularization and nutrient transport.
Keywords: DDMM, Guided Bone Regeneration, Porosity.
Topic: Health and Medicine