STANDARDIZATION AND ANTIDIABETES ACTIVITIES FROM ANTIHIPERTENSIVE JAMU
Aprilita Rina Yanti Eff, Sri Teguh Rahayu, Putu Gita Maya, M. Unggul Januarko
Departement of Pharmacy, Faculty of Health Sciences, Esa Unggul University Jakarta
Abstract
Jamu is a native Indonesian medicine that has been used since time ancient to overcome various diseases, including hypertension and diabetes mellitus. Standardization of herbal medicine represents a critical stage in the development of native Indonesian medicines. This study aims to standardize and assay the antidiabetic activity of antihypertensive jamu. Antihypertensive jamu was extracted by maceration using ethanol. Standardization includes non-specific parameters and specific parameters, assays of antidiabetic, antihypertensive, and antioxidant activity. Examination Non-specific parameters in the form of examination of water content, ash content, acid insoluble ash content, the level of substances dissolved in alcohol and water, Coliform microbial contamination, and mold/yeast rates. Specific parameters include organoleptic (color and texture), chemical content, identification of infrared spectrum, antioxidant activity with DPPH method, an alpha-glucosidase enzyme inhibitor, and Angiotensin-converting enzyme inhibitor activity. The results showed that antihypertensive jamu met the requirements of both specific and non-specific parameters, possessed activities of alpha-glucosidase enzyme inhibitor, Angiotensin-converting enzyme inhibitor and antioxidant with IC50 values of 49.95 ppm, 11.4 ppm, and 103.75 ppm respectively. Antihypertensive jamu meet the parameters of the requirements according to the Indonesian Herbal Pharmacopoeia standards and possess antihypertensive, antidiabetic, and antioxidant activities.
Keywords: Herbal medicine, antihypertensive, antidiabetic, standardization
Topic: Etnomedisin dan Etnofarmakologi