Effect of Catechin Isolates from Gambir (Uncaria gambir Roxb) on Fasting Blood Glucose and Glucagon Serum Level in Male Rats (Rattus norvegicus) Induced-by Alloxan
Rauza Sukma Rita (1*), Husna Yetti (2), Putra Santoso (3), Nita Afriani (4)
1)Department of Biochemistry, Faculty of Medicine, Universitas Andalas, Padang, Indonesia
Jalan Perintis Kemerdekaan No.94 Jati, Padang, Indonesia
*Corresponding author : (rauzasukmarita[at]med.unand.ac.id)
2) Department of Public Health and Community Medicine, Faculty of Medicine, Universitas, Padang, Indonesia
3) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, Indonesia
4) Department of Histology, Faculty of Medicine, Universitas Andalas, Padang, Indonesia
Abstract
Background : Hyperglycemia in diabetes melitus may leads oxydative stress and complications, either acute and chronic complications. Management of hyperglycemia is important to prevent bad effect of diabetes condition. Cathechin isolates from gambir (Uncaria gambir Roxb) consists of high amount of antioxidant and may help to prevent hyperglycemia. Purpose : This study aim to examine the effect of catechin isolates from Gambir (Uncaria gambir Roxb) on fasting blood glucose and glucagon serum level in rat induced-by alloxan. Methods : This study was an experimental research with Post Test Only Control Group Design and conducted on 35 male rats. Rats were divided into 5 groups. First group was considered as negative control group. Second group was considered as positive control group and induced by alloxan (150 mg/kg BW). Third, fourth, and fifth groups were considered as treated group 1, 2, and 3 (T1, T2, T3), also induced by alloxan, and were treated with different doses of catechin isolates for each group (10 mg/kg/day, 20 mg/kg/day, and 40 mg/kg/day). Catechin isolates were administered orally for 2 weeks. In the last day of experiment, fasting blood glucose and glucagon serum level were measured. Results : Catechin isolates decreased fasting blood glucose and glucagon serum level in rat induced-by alloxan significantly, but not in a dose dependent manner. Conclusion : Catechin isolates decreased blood glucose level by inhibiting glucagon serum level.
Keywords: cathecin isolates, alloxan, fasting blood glucose, glucagon, diabetes melitus
Topic: Communicable and Non Communicable Diseases