Potential of Secondary Metabolite Cromolaena odorata as Antibacterial Againts Pathogenic Bacteria
Endang Sulistyarini Gultom, Hasruddin, Meida Nugrahalia

Universitas Negeri Medan

Abstract

The purpose of this study was to isolate secondary metabolites from the ethanol extract of chirinyuh leaves (Cromolaena odorata) which have the potential to be anti-bacterial. The results of this study are expected to get an additional inventory of medicinal plants and in the long run add to the economic value of marketable herbal medicines. The planned method is to obtain extract by maceration of Harborn (1987) method, then the extract activity test which is highest in antibacterial was carried out. To test the antibacterial activity, a diffusion method is used. Furthermore, the extract which has the highest antibacterial activity was identified to be continued to the next stage. Based on the results obtained, the ethanol extract of the kirinyuh leaf contains secondary metabolites of flavonoids, alkaloids, tannins and saponins which have antibacterial activity. This was obtained from the ability of the extract to inhibit the growth of Staphylococcus aureus, Eschericihia coli, and Pseudomonas aeruginosa test bacteria with inhibitory zones of 12 mm, 16 mm, and 7 mm respectively. The MIC value of each test bacterium was different depending on the type of bacteria where the growth of Staphilococcus aureus was inhibited at a concentration of 6%, Eschericihia coli 3%, and Pseudomonas aeruginosa 12%.

Keywords: Secondary metabolites, Cromolaena odorata, Antibacterial, Chromatography.

Topic: Biology

Link: https://ifory.id/abstract-plain/WQ3HDn7UMTjg

Web Format | Corresponding Author (Endang Sulistyarini Gultom)