Phylogenetic Analysis of Masheer Fish from Indonesia based on mitochondrial 16S rRNA gene
Dwi Anggorowati Rahayu (1,2), Endik Deni Nugroho (2,3), Miftahul Mushlih (2,4), Rodiyati Azrianingsih (5), Nia Kurniawan (5)
1) Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, East Java, Indonesia
2) Indonesian Genetic and Biodiversity Community, Jl. Ikan Mujair No 15, Tunjung Sekar, Lowok Waru, Malang
3) Department of Biology, Borneo Tarakan University, North Kalimantan, Indonesia
4) Laboratory of Molecular Biology, Faculty of Healthy Science, Universitas Muhhamadiyah Sidoarjo
5) Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University
Abstract
Masheer is Indonesias endangered local fish. This study conducted to characterize and mapping the phylogenetic relationship of Masheer fish from East Java, West Kalimantan, Padang and North Sumatra using a partial sequence of mtDNA 16S rRNA. PCR amplicons at 542 bp in length. The construction of phylogenetic topology was made based on ML and NJ method using Kimura-2 parameter model. Based on the phylogenetic topology showed that Tor fish from Pasuruan are closely related to Tor duoronensis from Padang with a bootstrap value of 66%, while Tor duoronensis fish from North Sumatra and Tor tambra from West Kalimantan are in separated clusters. Sequence was compared and multiple sequence alignment revealed some polymorphic sites which can be used to differentiate Genus Tor from Indonesia. This 16S rRNA gene finding has a significant contribution to indicate phylogenetic of Masheers fish in Indoensia
Keywords: mitochondrial DNA, 16S rRNA, phylogenetic, genus Tor, polymorphic site
Topic: Bioconservation