STUDY OF SOYA ADDITION IN TRIS BASE EXTENDER ON THE QUALITY OF SENDURO GOATS SPERMATOZOA AND MEMBRANE INTEGRITY ON STORAGE
Nur Ducha, Widowati Budijastuti
Unesa
Abstract
Soya is an ingredient that is often added to semen extender as a component of macromolecules. This study aims to examine the effect of adding soy in the Tris base diluent on the quality and integrity of the Senduro goat spermatozoa membrane. Fresh semen was collected from the Senduro Goat using an artificial vagina, then observing the motility, viability, and integrity of the spermatozoa membrane. Fresh semen was diluted in tris base extender with different concentrations of soy, ie 0% (as a control group), 1%, 2%, and 3%. Stability of spermatozoa was analyzed based on the quality of spermatozoa, namely motility and viability, and membrane intebrity. Observation of motility and viability is done every day. Spermatozoa motility was observed under a microscope at 200 X magnification, by two people. Viability of spermatozoa was observed using negosin eosin staining. Spermatozoa membrane integrity was observed using the HOST method. The results showed the greatest motility, viability and integrity of the spermatozoa membrane at the beginning of storage were found in the tris + soya diluent of 2%. But at the end of storage, ie on the second day after storage, the quality of spermatozoa in the control group and the treatment group (2% and 3%) did not differ only. Based on the results of the study it can be concluded that the use of soy in the tris base diluent has not been able to provide optimal protection in Spermatozoa Senduro Goat druing storage at temperature of 4-5⁰C.
Keywords: spermatozoa od Goat Senduro, soya, tris base extender, temperature of 4-5⁰C
Topic: Biology