ANTIBACTERIAL AND CHARACTERIZATION OF SECONDARY METABOLITE COMPOUND FROM ETHYL ACETATE AND ETHANOL FRACTION of LEAVES Moringa oliefera L
Siti Rofida
University of Muhammadiyah Malang
Abstract
The microorganisms that cause infections can mutate due to excessive antibiotic exposure. One of the new drug search strategies is through exploration of active ingredients derived from plants that have been used empirically by the community. Moringa oleifera L leaf is a plant that has been used and has been shown to have antibacterial, antifungal, analgesic, and antihypertensive activities. Moringa oleifera L leaves contain secondary metabolites such as alkaloids, tannins, saponins, flavonoids, and phenols. The purpose of this study was to obtain active ingredients from the leaves of Moringa oleifera L which will be used as a Standardized Herbal Medicine product in the treatment of infectious cases. In order to get the active ingredient as an antibacterial, multilevel extraction is carried out using different polarity solvents, so that a fraction containing nonpolar, semipolar and polar compounds will be obtained. Antimicrobial potential will be tested on each fraction using the disk diffusion method. The results of identification of the compounds in the ethyl acetate fraction show the class of compounds Flavonoids, Terpenoids, Polyphenols and Anthraquinone while in the ethanol fraction Moringa oleifera L. leaves show the compounds of the compounds Alkaloids, Flavonoids, Terpenoids, Polyphenols and Anthraquinones while in the ethanol fraction Moringa oleifera L. , Polyphenols and Anthraquinone. Antimicrobial activity is shown in the ethyl acetate and ethanol fraction in both Staphylococcus aueus and Escherichia coli bacteria.
Keywords: Fractionation, Moringa oleifera leaves, secondary metabolite compounds, antibacterial
Topic: Pharmaceutical Science