DETECTION OF MYOGLOBIN PROTEIN IN GREEN SEATURTLE (Chelonia mydas) ORGANS WHICH CONSUMEHIGHLY CONCENTRATED O2 USING SPECTROPHOTOMETRY AND IMMUNODOT BLOTTECHNIQUES
Rini Puspitaningrum1*, Khadijah Dinda Y1, Erfan Handoko2
1Dept. of Biology, Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia
2 Dept of Physics. Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia
Abstract
Myoglobin is a hemoprotein with the important roleof being the binding and storage agent of oxygen in tissues. That is why it is important to study the existence of myoglobin in hypoxia tolerant animals, such as the green sea turtle (Chelonia mydas). This study aims to detect myoglobin protein in green sea turtle (Chelonia mydas) organs which consume highly concentrated O2 using spectrophotometry and immunodotblottechniques. In this study, the myoglobin protein of the heart, brain, and liver was analyzed using spectrophotometry at ƛ503nmand immunodot blot techniques with the antibody – anti myoglobin of the green sea turtle (Chelonia mydas). The results obtained is that the myoglobin protein is detected in all organs. The lungs are the organ with the lowest concentration of myoglobin protein from all the test organs. Based on the study results, it can be concluded that genetically, the green sea turtle (Chelonia mydas) would evolve as a tolerant animal toward hypoxia.
Keywords: myoglobin, hypoxia tolerant, green sea turtle (Chelonia mydas), organs which consume highly concentrated O2
Topic: Biology