HISTOPATHOLOGY of TESTIS in MALE MICE (Mus musculus albinus) POST GIVEN THE AQUEOUS LEAVES EXTRACT of NEEM (Azadirachta indica A. Juss)
Elsa Lisanti1, Dondin Sajuthi2, Muhammad Agil3, R Iis Arifiantini3, Adi Winarto3, Arwin4
1 Universitas Negeri Jakarta (UNJ) Department of Biology, Faculty of Mathematics and Natural Sciences, Kampus A, Gedung Hasjim Asjarie Lt. 9. Jl. Rawamangun Muka Jakarta Timur, 13220, Indonesia
2 Department of Veterinary Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine, Institut Pertanian Bogor (IPB), Dramaga Bogor 16680, Indonesia)
E-mail : sajuthi[at]indo.net.di
3 Departement Anatomy, Physiology, and Pharmacology,Faculty of Veterinary Medicine, Institut Pertanian Bogor (IPB), Dramaga Bogor 16680, Indonesia
4 Center Isotopes Radiation Application, National Nuclear Energy Agency of Indonesia. Jl. Lebak Bulus Raya No. 49 Jakarta Selatan, Indonesia
Abstract
Neem (family Meliaceae) is non-leguminous multipurpose plant has antispermatogenic effect in animals and male men. Objectives: this research to assess and evaluate the effect of aqueous leaves extract of neem on the development of spermatogenic cell of male mice. Methods: Male mice were divided into five groups. Group I as control (C1-1) with the absence of treatment. Group II and III were orally treated by aqueous leaves extract of neem at doses 0.25 and 0.50 mg/kg bw for 36 days. Group IV and V were treated by administering aqueous leaves extract of neem at doses 0.25 and 0.50 mg/kg BW for 36 days followed with another 36 days with no treatment. Results: neem leaves extracts to cause decrease of spermatogenic cell (spermatogonia, spermatocyte, and spermatid) (p<0.05). Observation of testicular histology in treated mice showed degenerative changes that are not homogeneous in the seminiferous tubules. Tubular mice treated showed loss of the germinal epithelium, and reduction in the number of germ cells (spermatogonia, spermatocyte, and spermatid) in the testes of treated mice. After 36 days of stopped of treatment, the number of germ cells had not recovered as control (p <0.05). Conclusion: These results indicate that the aqueous extract of neem leaves is irreversible to testicular histopathology and decreases the development of spermatogenic cells in male mice.
Keywords: Aqueous; Leaves; Mice; Neem; Testis
Topic: Biology