The use of 2,4-D and thidiazuron for induction of kopyor coconut callus
Desi Maulida (a), Lisa Erfa (b), Hilman Hidayat (c), Rizka Novi Sesanti (d)
a) Horticulture, Politeknik Negeri Lampung
Jl. Soekarno Hatta No 10 Rajabasa Bandar Lampung
Abstract
The low production of kopyor coconuts is caused by kopyor-producing coconut plants that only rely on kopyor-bearing coconut trees that have heterozygot genetic constitution (Kk) and are grown from normal phenotypic coconut seeds. Kopyor fruit cannot be grown naturally. One alternative to get true to type kopyor seeds is by embryo culture. Development of reliable clone propagation such as somatic embryogenesis will quickly provide embryos for kopyor. From one kopyor em-bryo that is produced will get many somatic embryos which can then become kopyor coconut seeds. The aim of this research is getting an effective method to produce true to type kopyor seeds and knowing the influence of 2,4-D with or withaout TDZ in inducing callus to produce somatic embryos. This research was carried out in the Lampung State Polytechnic laboratory using a factorial. Factor 1 is 2,4-D concentration 0, 2,5, 5, 7.5, and 10 mg/l with or without the addition of 0.5 mg/l. Further data analysis using the 5% LSD test. The parameters observed in this study were: 1) Percentage of explants callus 2) Callus diameter, 3) Callus weight. The results showed that Increasing concentrations of 2,4-D and 7.5 mg/l can increase the percentage of embryogenic callus, callus diameter, and weighing callus. The addition of TDZ concentration of 0.5 to the medium 2,4-D resulted in a decrease in the percentage of embryogenic callus, callus diameter is narrower and lower weight on embryonic callus kopyor coconut. The concentration of 2,4-D best to induce callus on embryo kopyor is 7.5 mg/l.
Keywords: Kopyor, 2,4-D, TDZ
Topic: Agriculture Engineering and Biotechnology