Telomerase Content (TERT) in Limphocytesof White Rats (Rattus norvegicus)in Different Ages
Rini Puspitaningrum1*, Mohamad Sadikin2, Adiati Bintari Ayu1
1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Indonesia 10520
2 Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Indonesia
Abstract
Aging getting more attention in meetings both in between scientists as public. Long telomeres on lymphocytes able predict the aging process and related to change telomerase function. This research aimed to measure levels telomerase enzyme on lymphocytes cell with age 6-8 weeks and 2-3 years. This research used experimental method. This researchwas conductedon February-May 2018 in Laboratory Biochemistry and Biology Molecular Faculty Medical University of Indonesia. Based on the research that has been done, the level of telomerase enzyme in Rattusnorvegicus lymphocytes within age 6-8 weeks had an average 2.24 ± 0.09 pg / mL lymphocyte. Telomerase enzyme levels in lymphocytes aged 2-3 years had an average 2.27 ± 0.15 pg / mL.This research obtained accurate information that can be used to find out each individual who doesn-t show the same level at the same time, was not statistically significantly different. This is an interesting thing to do for a more heterogeneous environment and by paying attention to individuals per individual from various daily activities.
Keywords: Aging , telomerase enzyme, lymphocytes, cell degeneration
Topic: Biology