The Chronic Effects of Aqueous Fraction of Lemon Administration on Body Weight and Visceral Fat Mass of Old Mice Fed by High-fat Diet
Annisa Rahmah Furqaani (a*), Retno Ekowati (a), Heriansyah (b), Arief Budi Yulianti (a), Maya Tejasari (a), Miranti Kania Dewi (c)
(a) Histology and Biomedical Science Departement, Faculty of Medicine, Universitas Islam Bandung
(b) Bachelor of Medicine, Faculty of Medicine, Universitas Islam Bandung
(c) Pharmacology Departement, Faculty of Medicine, Universitas Islam Bandung
Abstract
Lemon (Citrus limon) contains various kinds of flavonoids that have beneficial effects in modulating lipid profile and controlling obesity. The aim of this study was to determine the chronic effects of aqueous fraction of lemon administration on body weight and visceral fat mass of old mice fed by high-fat diet. Twenty-six female mice (Mus musculus) DDY strains (45-50 weeks in age; 40-50 g in weight) were divided into 5 groups, group 1 (K1) were fed by high-fat diet (PTL); group 2 (K2) were fed by standard diet; and three other groups that fed by PTL and got aqueous fraction of lemon with 3 different doses, 0.20 g/KgBB (K3), 0.40 g/KgBB (K4), and 0.80 g/ KgBB (K5). Body weight measurements were taken before and at the end of the treatment period. After that, the mice were sacrificed to be measured visceral fat mass. The results showed that final weight and visceral fat mass of K3 was significantly lower compared to other groups (p <0.05). These results indicate that the aqueous fraction of lemon has the potential to control obesity by reducing the mass of visceral fat, but its mechanism of action depends on the dose of administration.
Keywords: Aqueous fraction, body weight, lemon, visceral fat mass
Topic: Basic and Clinical Medical Sciences