Encapsulation of Nanoparticle Fe3O4 with Phospholipid and Dimethylaminobenzyl-Chitosan as Protein β-Amyloid Contrast Agent
Husaini Ardy(1), Megantara Pura (1), Agus Jatmiko(1), Arie Wibowo (1,2), Bambang Sunendar Purwasasmita(3*)
(1) Department of Materials Engineering, Faculty of Mechanicals and Aerospace Engineering, ITB
(2) Research Center for Nanoscience and Nanotechnology, ITB
(3) Department of Engineering Physics, Faculty of Technological Industry, ITB (*Corresponding author: purwa[at]tf.itb.ac.id)
Abstract
Alzheimer is a disease that slowly destroys memory and cognitive ability. There are 46 million Alzheimer patients around the world and 22 million of them are in Asia. The number of Alzheimer’s patients are expected to increase fourfold in 2050. Magnetic Resonance Imaging (MRI), with contrast agent can be used as early detection of this disease. However, enzyme ligand that used for MRI media contrast is neurotoxic. On the other hand, research on PET contrast agent used dimethylaminobenzyl ligand has not widely studied yet. This contrast agent is a promising alternative of ligand because it will be able to interact with Amyloid protein. The strategy of this research is to design magnetite-based MRI contrast coated with dimethylaminobenzyl-chitosan as its ligand. The modified chitosan showed a new peak at 353 nm in UV-Vis result compared with normal chitosan. This result proved that chitosan was modified by dimethylaminobenzaldehyde. FTIR spectrum of encapsulated nanoparticles indicated strong spectrum of iron oxide and 1,4 disubstituted benzene. XRD analysis shows that the nanoparticle formed is D-Magnetite with crystal size 13 + 4 nm. Lastly TEM image shows dark spheres inside the membrane. While, XRD analysis result showed that the size of dark sphere is 12 + 4 nm, which is in range of crystal size for magnetite. Gyration radius of modified chitosan is 6 nm and very close to the thickness of the membrane. This result proved that the modified chitosan is encapsulating the magnetite.
Keywords: Alzheimer, Amyloid-β, β-sheet, MRI contrast agent, Fe3O4 nanoparticles, modified chitosan
Topic: Nano Science and Technology