REMOVAL OF COPPER METAL (Cu2+) BY MIXED BACTERIA CULTURE Thiobacillus sp. AND Clostridium sp. WITH VARIATIONS OF TEMPERATURE AND CONCENTRATION OF POLLUTANT IN LIQUID MEDIA
Anna Kristina Rosa Vernans, Bambang Iswanto, Astri Rinanti
Universitas Trisakti
Abstract
Bioremediation is an effective alternative for removing heavy Cu2+ metals because it is environmentally friendly, a cost-effective technology and permanently detoxifies pollutants. This research was conducted to remove heavy Cu2 + metals using the bioremediation method by utilizing a mixed culture of Thiobacillus sp. and Clostridium sp. as bioremedian. This research was conducted on liquid media which was started in artificial growth media is Stone Mineral Salt solution (SMSs) with the parameters tested in the form of temperature (oC) and Cu2+ heavy metal pollutant concentration. The variabels used consisted of temperature (oC) is 20, 25, 30 and 35, pollutant concentration (ppm) is 25, 50, 75 and 100. Analysis by Atomic Absorption Spectrophotometry (AAS) was carried out to determine the heavy metal content of Cu2+. In addition, the calculation of Cu2 + heavy metal removal efficiency was carried out. In this research, the exponential phase of the growth of mixed culture of bacteria was at a temperature of 30 oC, pH of 7 with a pollutant concentration of 100 ppm. So that, the percent allowance for heavy metals Cu2 + in liquid media until 90%. The efficiency of the use of mixed cultures of the bacteria Thiobacillus sp. and Clostridium sp. as bioremedian in reducing the heavy metal content of Cu2+ in liquid media it can reach the range until 90% with the variation used is a temperature of 30 oC, pH of 7, the pollutant concentration of 100 ppm with the contact time used is 48 hours.
Keywords: Bioremediation, Heavy metal Cu2+, Thiobacillus sp., Clostridium sp., Removal, Growth Media
Topic: Environmental Engineering