The effect of suplementation lignolitik probiotic for digestibility and efficiency of microbial protein synthesis using in vitro residual gas production
Indah Prihartini1 and Miftachi Ari2
University of Muhammadiyah Malang
Abstract
Based on this research from Prihartini, (2007a) found three isolates lignochlorin that have high potential in growth, production and enzyme activity, and specifically degrade lignin and organochlorin, grow well in the media are natural carriers of rice straw. But each also has the capability of microbial biodegradation of lignin is different, it is influenced by the composition and structure of lignin in plant cell walls and the ability lignolitik oxidative enzymes produced by microbes (Prihartini, 2008). Third lignochloritik bacterial isolates that have the nature of facultative anaerobic bacteria that can live in both aerobic and anaerobic conditions also can live inside or outside the body of cattle, bacterial growth is also high so that it can be used as probiotics and can be introduced into the rumen to improve the digestibility of nutrients in the rumen and the ability to digest lignocellulosic material (Prihartini, 2007b). The aims of this research were to know the effect of supplementation lignolitic probiotic in rice straw on gas production, digestibility and efficiency of microbial protein synthesis using in vitro residual gas production and to know using the optimal lignolitic probiotic in rice straw The method used was Randomized Block Design (RBD) with 4 treatments and 3 groups. The treatments were P0: rice straw + 0% probiotic level; P1: rice straw + 0.5% probiotic level; P2: rice straw + 1.0% probiotic level; and P3: rice straw + 1.5% probiotic level. In-vitro gas production was observed at incubated for 2. 4. 8. 16. 24. 36. 48. 72 and 96 hours, digestibility and efficiency of microbial protein synthesis using in vitro residual gas production were observed at 96 hours incubation period. The result showed that treatment high significantly effected (P<0.01) on gas production, digestibility and efficiency of microbial protein synthesis. The gas production at 96 hours incubation for P3 (73.86 ml/500mg DM) was higher than for control P0 (67.38 ml/500mg DM) and another treatment. The dry matter and organic matter digestibility at the end of incubation for P3 (43.56% and 48.41%) were higher than for control P0 (33.80% and 37.56%) and another treatment. The highest efficiency of microbial protein synthesis was P3 (34.26 g N/kg BOTR) at 96 hour incubation. Degradation of lignin in the rumen is very limited and only a small portion of rumen microbial compound that has the ability to transform not condensed lignin (Kanahau, 2005). Presence of laccase enzyme in the lignolitic probiotic, was able to break the bond of lignocellulose in the rumen which increases digestibility. Residue digestibility of gas production is dry matter and organic matter fermented in the rumen to use for microbial synthesis protein. Based on the results we can conclude that: Addition of lignolitic probiotics in rice straw can increase gas production, dry matter digestibility, organic matter digestibility and efficiency of microbial protein synthesis at 96 hou
Keywords: lignolitic probiotic, invitro, microbial protein synthesis
Topic: Environmentally Sustainable Agriculture