Development of Reusable Microfluidic Chamber as a Sample Container for Sensor Platform
Marvel Sugi Hartono (a*), Lavita Nuraviana Rizalputri (a), Raih Rona Althof (a), Isa Anshori (a) and Brian Yuliarto (b)(c)
a) Department of Biomedical Engineering, School of Electrical Engineering and Informatics, Institut Teknologi Bandung,
*marvelsugi97[at]gmail.com
Jl. Ganesha 10, Bandung 40132, Indonesia
b) Department of Engineering Physics, Faculty of Industrial Technology, Institut Teknologi Bandung,
Jl. Ganesha 10, Bandung 40132, Indonesia
c) Research Center of Nanoscience and Nanotechnology, Institut Teknologi Bandung,
Jl. Ganesha 10, Bandung 40132, Indonesia
Abstract
Microfluidic is a technology capable of manufacturing and miniaturize a device with a chamber inside, hence, fluids can go through. Concept of microfluidic brought an integrated system in a micro size platform that can operates as well as conventional laboratory. In this project, microfluidic was used as a platform for a sample to react with electrodes placed inside the microfluidic. Our work is interesting as our platform can be cleaned and reuse again. Microfluidic was made by using PDMS (Polydimethylsiloxane) and its curing agent. The container for microfluidic resin was made by using acrylic that has been laser cut. The chamber (master mold) for microfluidic was designed from polyimide tape and then being cut by using cutting sticker machine. Microfluidic platform composed of two sides which held together by magnets placed around it. The fluidic test was conducted by injecting liquids through the inlet using micropipette. During the process, we observed whether there is any leakage in the microfluidic. Microfluidic can be cleaned by opening its layer and wipe it out using distilled water and ethanol. To find out whether microfluidic does not affect the electrochemical measurements of the electrodes, a comparison of cyclic voltammetry (CV) results when using and not using microfluidic was being done. It was found that the changes that occurred were not noticeable. In addition, stability tests and the effect of scan-rate changes on the CV curves are also performed to prove that the electrodes and microfluidics can be an intact platform for measurement.
Keywords: microfluidic, miniaturize, PDMS, reusable, Lab on Chip, biosensor platform
Topic: Biomedical, Robotic and ICT engineering