Larva Serum Antigen-G of Musca domestica as Immunoglobulin Production Promotor in Goats
Laurentius J.M. Rumokoy (a). Ivonne M. Untu (b).Santi Turangan (b). Wisje Lusia Toar (b*)
a) Entomology Program, Postgraduate School, Sam Ratulangi University, Jl. Kampus Unsrat. Manado 95115, Indonesia
b) Animal Science Program, Faculty of Animal Husbandry, Sam Ratulangi University, Jl. Kampus Unsrat. Manado 95115, Indonesia
*wisje_toar[at]live.com
Abstract
This experiment aimed to study the effect of serum G-antigen on M. domestica insect larvae (LAS) as promoter antigen on serum immunoglobulin production in organically managed goat livestock. This study used 12 local goat animals which were divided into two groups, a control group and a group receiving treatment. Insect rearing was used to obtain larvae, the antigen-G was then extracted from the larvae to be used as promoter antigen to enhance the serum antibody production which was subcutaneously immunized in experimental goats and incubated for a period of 14 days. Blood collection of 2.5 ml was taken through the jugular vein and then quantification of the total antibody is carried out. The data of the LSA extract proportion level were statistically analyzed with t-test, and the quality classification level of serum immunoglobulin of animals groups were statistically analysed. The results showed that the serum of animal treated with LSA of M. domestica resulted in a higher level of immunoglobulin (P <0.01) compared to the control. We conclude that the antigen-g substance (LSA) could support the efforts to improve the production of organic goats livestock by increasing the total level of antibodies circulating in the blood.
Keywords: Insect, Musca domestica, antigen, antibody, goats
Topic: General animal production and husbandries (ruminants and non-ruminants)