Antibacteria activity of bacteria associated with healthy Acropora formosa against white plague found in Acropora formosa from Gili Labak Island-Madura-Indonesia
Insafitri, Nur Hidayatullah, Wahyu Andy Nugraha
Department of Marine Science, Universitas Trunojoyo Madura, Bangkalan, Indonesia 69162
E-mail: insafitri[at]trunojoyo.ac.id
Abstract
Gili Labak has a good coral reef condition, but has a lot of problem including coral disease. This study aims to identify bacteria that are associated with Acropora formosa corals which have the potential as antibacterial white plague disease in Acropora sp. The method used in the antibacterial test is the overlay test and agar diffusion test, and laboratory analysis using bacterial isolation, DNA extraction, DNA amplification, squencing and phylogenetic analysis. Based on bacteria morphology, four bacterial isolates were found from healthy Acropora, namely AF1, AF2, AF3, and AF4. From the results of the antibacterial activity test using the pathogenic bacterium Bacillus firmus, isolates AF1 produced a inhibition zone in three days of 5.10 ± 0.10 mm, 2.7 ± 0.04 mm, and 3.80 ± 0.02 mm, respectively, while AF3 produced a inhibition zone of 2.90 ± 0.16 mm, 3.10 ± 0.06 mm, and 2.7 ± 0.11 mm, respectively. Meanwhile, the antibacterial activity test using pathogenic bacteria Bacillus dretensis, AF2 produced a inhibition zone of 3.80 ± 0.01 mm, 2.20 ± 0.15 mm, and 1.30 ± 0.09 mm, respectively, while AF4 produced a inhibition zone of 4.60 ± 0.060 mm, 2.90 ± 0.06 mm, and 1,00 ± 0.06 mm, respectively. The phylogenetic analysis showed that AF1, AF3 and AF4 had 99% similarity with Virgibacillus salarius, and AF2 had 98% similarity with Halomonas sp.
Keywords: coral disease; white plague; acropora formosa; antibacterial
Topic: Bioconservation